TIM-4 Antibody (921832) [Alexa Fluor® 405] Summary
Immunogen |
Mouse myeloma cell line NSO-derived recombinant human TIM-4 Glu25-Leu315 Accession # Q96H15 |
Specificity |
Detects human TIM-4 in direct ELISAs.
|
Isotype |
IgG1 |
Clonality |
Monoclonal |
Host |
Mouse |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Flow Cytometry 0.25-1 ug/10^6 cells
|
Packaging, Storage & Formulations
Storage |
Store the unopened product at 2 - 8 °C. Do not use past expiration date. |
Buffer |
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide. |
Notes
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for TIM-4 Antibody (921832) [Alexa Fluor® 405]
Background
TIM-4 (T cell; immunoglobulin; mucin-4), also known as SMUCKLER, is a 60 kDa member of the TIM family of immune regulating proteins. TIMs are type I transmembrane proteins with one Ig-like V domain and one Ser/Thr-rich mucin domain (1 - 3). The human TIM-4 cDNA encodes a 378 amino acid (aa) precursor that includes a 24 aa signal sequence, a 290 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 43 aa cytoplasmic tail (4). Structurally, TIM-4 is distinguished from other TIMs by the presence of an RGD motif in its Ig domain and the lack of a site for tyrosine phosphorylation in its cytoplasmic tail. The mucin domain in TIM-4 is larger than in TIM-1 or TIM-3. Within the ECD, human TIM-4 shares 35% and 23% aa sequence identity with TIM-1 and TIM-3, respectively. A TIM-2 ortholog has not been identified in human. The ECD of human TIM-4 shares 45% aa sequence identity with that of mouse and rat TIM-4. TIM-4 is expressed by macrophages and mature dendritic cells but not by lymphocytes (4, 5). TIM-4 binds specifically to TIM-1 which is also the cellular receptor for the hepatitis A virus, and has been implicated in the development of asthma (5 - 7). Among hematopoietic cells, TIM-1 is expressed on activated B and T cells, preferentially in the Th2 subset of CD4+ T cells (5, 8). The interaction of TIM-4 with TIM-1 induces costimulatory and hyperproliferative signals in T cells (5).
- Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454.
- Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
- Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362.
- Shakhov, A.N. et al. (2004) Eur. J. Immunol. 34:494.
- Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
- Feigelstock, D. et al. (1998) J. Virol. 72:6621.
- McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
- Khademi, M. et al. (2004) J. Immunol. 172:7169.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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