Western Blot: Thyroid Peroxidase Antibody [NBP2-27544] - (0.1 ug/ml) Analysis of Thyroid Gland lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody [NBP2-27544] - Staining of Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
WB: Approx. 110 kDa band observed in human thyroid gland lysates (calculated MW of 103 kDa band according to NP_000538.3). IHC-P: Human thyroid gland shows textured staining in the cytoplasm of epithelial cells.
Publications
Read Publications using NBP2-27544 in the following applications:
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified
Alternate Names for Thyroid Peroxidase Antibody
EC 1.11.1
MSA
TDH2A
thyroid microsomal antigen
thyroid peroxidase
thyroperoxidase
TPXEC 1.11.1.8
Background
Thyroid Peroxidase encodes a membrane-bound glycoprotein. The encoded protein acts as an enzyme and plays a central role in thyroid gland function. The protein functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Multiple transcript variants encoding distinct isoforms have been identified for this gene. Additional splice variants have been described but their biological natures have not been determined. [provided by RefSeq]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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