TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free Summary
| Immunogen |
PHA activated rat lymphocytes. |
| Isotype |
IgG2a |
| Clonality |
Monoclonal |
| Host |
Mouse |
| Gene |
TFRC |
| Purity |
Protein G purified |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
- CyTOF-ready
- Flow (Intracellular)
- Flow Cytometry 1:50-1:200
- Immunocytochemistry/ Immunofluorescence 1:10-1:500
- Immunohistochemistry 1:10-1:500
- Immunohistochemistry-Frozen 1:10-1:500
- Immunohistochemistry-Paraffin 1:10-1:500
- Western Blot 1:100-1:2000
|
| Application Notes |
This antibody is CyTOF ready. |
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer |
PBS |
| Preservative |
No Preservative |
| Concentration |
1 mg/ml |
| Purity |
Protein G purified |
Alternate Names for TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free
Background
OX26 TfR clone is useful in studies of dividing haematopoietic and tumour cell populations, and metabolic activity. In comes in a variety of pre-conjugated fluorophores for histochemical and flow applications.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Product General Protocols
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Video Protocols
FAQs for TfR (Transferrin R) Antibody (NBP2-80976) (0)
![Western Blot: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976] - Total protein from rat adrenal gland cells (PC12) and rat placenta was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-CD71 in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-OX26-Azide-and-BSA-Free-Western-Blot-NBP2-80976-img0001.jpg "Western Blot: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976] - Total protein from rat adrenal gland cells (PC12) and rat placenta was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-CD71 in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976] - PC-12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti TfR/Transferrin R (OX26) NB200-585 at a 1:100 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (red) at a 1:200 dilution. Nuclei were counterstained with DAPI (blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-OX26-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80976-img0002.jpg "Immunocytochemistry/Immunofluorescence: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976] - PC-12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti TfR/Transferrin R (OX26) NB200-585 at a 1:100 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (red) at a 1:200 dilution. Nuclei were counterstained with DAPI (blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.")
![Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976] - Analysis of FFPE mouse spleen using TfR (OX26) antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenchi](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-OX26-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80976-img0003.jpg "Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976] - Analysis of FFPE mouse spleen using TfR (OX26) antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenchi")
![Flow Cytometry: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976] - Staining of rat spleen lymphocytes with mouse anti rat CD71. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-OX26-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80976-img0007.jpg "Flow Cytometry: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976] - Staining of rat spleen lymphocytes with mouse anti rat CD71. Image from the standard format of this antibody.")
![Flow Cytometry: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976] - An intracellular stain was performed on PC12 cells with TfR (Transferrin R) [OX26] Antibody NB200-585AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-OX26-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80976-img0004.jpg "Flow Cytometry: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976] - An intracellular stain was performed on PC12 cells with TfR (Transferrin R) [OX26] Antibody NB200-585AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.")
![Flow Cytometry: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976] - Analysis using the Biotin conjugate of NB200-585. Staining of mouse spleen.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-OX26-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80976-img0005.jpg "Flow Cytometry: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976] - Analysis using the Biotin conjugate of NB200-585. Staining of mouse spleen.")
![Flow Cytometry: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976] - Staining of rat spleen cells with mouse anti rat CD71: RPE. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-OX26-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80976-img0006.jpg "Flow Cytometry: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976] - Staining of rat spleen cells with mouse anti rat CD71: RPE. Image from the standard format of this antibody.")
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