![Western Blot: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Western Blot Analysis of Jurkat, Raji, and THP-1 cell lysate using TfR (Transferrin R) Antibody (DF1513).](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-DF1513-Western-Blot-NBP2-32945-img0006.jpg "Western Blot: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Western Blot Analysis of Jurkat, Raji, and THP-1 cell lysate using TfR (Transferrin R) Antibody (DF1513).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Simple Western, Flow, ICC/IF |
| Clone | DF1513 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34602) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Additional Information | Clone DF1513 was used by HLDA to establish CD designation. |
| Immunogen | KG1 acute myeloid leukemia cell line |
| Localization | Cell surface |
| Specificity | It recognizes a ~90-95kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa (Workshop IV). This monoclonal antibody is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TFRC |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Optimal dilution for a specific application should be determined. |
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| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Verified Customer |
WB | Canine and Feline | 06/29/2017 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
Research Areas for TfR (Transferrin R) Antibody (NBP2-32945)Find related products by research area.
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Transferrin and the blood brain barrier Transferrin, an iron binding protein that facilitates iron uptake in cells, is an integral part of a mechanism that may introduce antibody therapies to the central nervous system. Currently, the brain’s ability to take in antibody therapies i... Read full blog post. |
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| Verified Customer 06/29/2017 |
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| Application: | WB | |
| Species: | Canine and Feline |
![Immunocytochemistry/Immunofluorescence: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Immunofluorescence Analysis of Human Jurkat cells labeling CD71 with TfR (Transferrin R) Antibody (DF1513) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-DF1513-Immunocytochemistry-Immunofluorescence-NBP2-32945-img0005.jpg "Immunocytochemistry/Immunofluorescence: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Immunofluorescence Analysis of Human Jurkat cells labeling CD71 with TfR (Transferrin R) Antibody (DF1513) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)")
![Flow Cytometry: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Flow Cytometric Analysis of human Jurkat cells using TfR (Transferrin R) antibody (DF1513) followed by Goat anti-Mouse IgG-CF488 (Orange); cells alone (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-DF1513-Flow-Cytometry-NBP2-32945-img0007.jpg "Flow Cytometry: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Flow Cytometric Analysis of human Jurkat cells using TfR (Transferrin R) antibody (DF1513) followed by Goat anti-Mouse IgG-CF488 (Orange); cells alone (Blue); Isotype Control (Red).")
![Simple Western: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Simple Western lane view shows a specific band for TfR (Transferrin R) in 0.2 mg/ml of Jurkat (left) and K562 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-DF1513-Simple-Western-NBP2-32945-img0001.jpg "Simple Western: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Simple Western lane view shows a specific band for TfR (Transferrin R) in 0.2 mg/ml of Jurkat (left) and K562 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Simple Western: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Electropherogram image of the corresponding Simple Western lane. TfR (Transferrin R) antibody was used at 10 ug/ml dilution of Jurkat and K562 lysates(s) respectively.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-DF1513-Simple-Western-NBP2-32945-img0002.jpg "Simple Western: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Electropherogram image of the corresponding Simple Western lane. TfR (Transferrin R) antibody was used at 10 ug/ml dilution of Jurkat and K562 lysates(s) respectively.")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")
