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Recombinant Human TFF3 Protein

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SDS-Page: Human TFF3 Protein [NBP2-35060]
SDS-Page: Human TFF3 Protein [NBP2-35060]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, Bioactivity
Format
Carrier-Free
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier-Free
NBP2-35060-1mg
Carrier-Free
NBP2-35060-100ug
Carrier-Free
NBP2-35060-500ug
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Recombinant Human TFF3 Protein Summary

Description
A homodimeric protein consisting of two 59 amino acid chains corresponding to TFF3, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds Source: E. coli

Uniprot ID: Q07654

Amino Acid Sequence: EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK PLQEAECTF

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
Details of Functionality
Trefoil Factor 3 protein is fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10 ug/ml, corresponding to a specific activity of > 100 IU/mg.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
TFF3
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Note
Less than 1 EU/ug of TFF3 as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • SDS-Page
Theoretical MW
13.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative
No Preservative
Concentration
LYOPH
Purity
> 97 % pure by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Alternate Names for Recombinant Human TFF3 Protein

  • HITF, human intestinal trefoil factor10Intestinal trefoil factor
  • ITF
  • TFF3
  • TFI
  • trefoil factor 3 (intestinal)

Background

Trefoil Factor 3 (TFF3), also known as ITF and P1.B, plays an important role in epithelial regeneration and wound healing. It is expressed by epithelial goblet cells in the respiratory tract, biliary and breast ducts, small and large intestine, and cardia of the stomach. It is upregulated in response to a range of gastrointestinal epithelial disruptions and induces the migration of biliary, bronchial, and intestinal epithelial cells. Its upregulation is also associated with and enhances tumor cell invasion and metastasis. TFF3 promotes angiogenesis and enhances VEGF upregulation in tumor cells. It can form disulfide-linked dimers or associate into disulfide-linked complexes with other intestinal mucous proteins including FCGBP and MUC-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol TFF3
Uniprot