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TFF3 Antibody [Unconjugated]

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Western blot shows lysates of human pancreas tissue and KATO-III human gastric carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human TFF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4407) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TFF3 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human pancreas tissue and KATO-III human gastric carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human TFF3 Antigen Affinity-purified Polyclonal Antibody (Catalog ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF4407
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF4407-SP
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TFF3 Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TFF3
Ala21-Phe80
Accession # Q07654
Specificity
Detects human Trefoil Factor 3 (TFF3) in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) TFF1 and rhTFF2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
TFF3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publication using
AF4407 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TFF3 Antibody [Unconjugated]

  • HITF, human intestinal trefoil factor10Intestinal trefoil factor
  • ITF
  • TFF3
  • TFI
  • trefoil factor 3 (intestinal)

Background

TFF3 (Trefoil factor 3; also hP1.B and intestinal trefoil factor) is a 7 kDa member of the trefoil factor family of protease-resistant peptides. It is expressed by multiple epithelial cell types, acts in an autocrine or paracrine manner and contributes to epithelial cell migration and survival. Mature human TFF3 is 59 amino acids (aa) in length (aa 22-80). It contains one P (or trefoil) domain that is characterized by the presence of three cysteine bond-mediated loops (aa 30-72). Human TFF3 exists as both a monomer and disulfide-linked dimer. There are three potential isoform variants. One shows an alternate start site at Met8, a second shows a 50 aa N‑terminal extension and a third shows a 109 aa substitution for aa 29-80. Over aa 21-80, human TFF3 shares 77%, 68%, and 72% aa identity with mouse, canine, and rat TFF3, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TFF3 Antibody (AF4407)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ELISA Development.


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Bioinformatics

Gene Symbol TFF3
Uniprot