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Testin Antibody

Images

 
Independent Antibodies: Immunohistochemistry-Paraffin: Testin Antibody [NBP1-87987] - Staining of human endometrium, liver, testis and urinary bladder using Anti-TES antibody NBP1-87987 (A) shows similar protein ...read more
Orthogonal Strategies: Western Blot: Testin Antibody [NBP1-87987] - Analysis in human cell lines A-431 and U-251MG. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Independent Antibodies: Western Blot: Testin Antibody [NBP1-87987] - Analysis using Anti-TES antibody NBP1-87987 (A) shows similar pattern to independent antibody NBP1-85072 (B).
Immunocytochemistry/ Immunofluorescence: Testin Antibody [NBP1-87987] - Staining of human cell line SiHa shows localization to cytosol & focal adhesion sites. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Testin Antibody [NBP1-87987] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Testin Antibody [NBP1-87987] - Staining of human endometrium moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Testin Antibody [NBP1-87987] - Staining of human liver shows no cytoplasmic positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Testin Antibody [NBP1-87987] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Testin Antibody [NBP1-87987] - Staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.
Immunohistochemistry-Paraffin: Testin Antibody [NBP1-87987] - Staining of human liver shows no positivity in hepatocytes as expected.
Simple Western: Testin Antibody [NBP1-87987] - Simple Western lane view shows a specific band for TES in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more
Simple Western: Testin Antibody [NBP1-87987] - Electropherogram image(s) of corresponding Simple Western lane view. Testin antibody was used at 1:20 dilution on RT-4 lysate(s).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Testin Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: QLPAHDQDPSKCHELSPREVKEMEQFVKKYKSEALGVGDVKLPCEMDAQGPKQMNIPGGDRSTPAAVGAMEDKSAEHKRTQYSCYCCKLSMKEGDPAIYAERAGYDKLWHPACFVCSTCHELLVD
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TES
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Simple Western reported by internal validation
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in IHC-P reported in scientific literature (PMID:34440214).
Control Peptide
Testin Protein (NBP1-87987PEP)
Publications
Read Publications using
NBP1-87987 in the following applications:

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%), Rat (85%). Reactivity reported in scientific literature (PMID: 23227862)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Testin Antibody

  • DKFZp586B2022
  • MGC1146
  • TESS
  • TESS-2
  • TESTIN
  • testis derived transcript (3 LIM domains)

Background

Cancer-associated chromosomal changes often involve regions containing fragile sites. This gene maps to a commom fragile site on chromosome 7q31.2 designated FRA7G. This gene is similar to mouse Testin, a testosterone-responsive gene encoding a Sertoli cell secretory protein containing three LIM domains. LIM domains are double zinc-finger motifs that mediate protein-protein interactions between transcription factors, cytoskeletal proteins and signaling proteins. Multiple protein isoforms are encoded by transcript variants of this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Testin Antibody (NBP1-87987)(4)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TES