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TEAD1 Antibody - Azide and BSA Free

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Western Blot: TEF1 Antibody [NBP2-95204] - Analysis of extracts of various cell lines, using TEF1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 120s.
Western Blot: TEF1 Antibody [NBP2-95204] - Analysis of extracts of various cell lines, using TEF1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per ...read more
Immunocytochemistry/ Immunofluorescence: TEF1 Antibody [NBP2-95204] - Analysis of MCF7 cells using TEF1 .
Chromatin Immunoprecipitation: TEF1 Antibody [NBP2-95204] - Analysis of extracts of HeLa cells, using TEF1 rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-95204-0.02ml
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TEAD1 Antibody - Azide and BSA Free Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 135-215 of human TEAD1 (NP_068780.2). AIHNKLGLPGIPRPTFPGAPGFWPGMIQTGQPGSSQDVKPFVQQAYPIQPAVTAPIPGFEPASAPAPSVPAWQGRSIGTTK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TEAD1
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 1:50-1:200
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1:100 - 1:500

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.05% Proclin 300
Purity
Affinity purified

Alternate Names for TEAD1 Antibody - Azide and BSA Free

  • AA
  • NTEF-1
  • Protein GT-IIC
  • REF1
  • TCF13
  • TCF-13
  • TEA domain family member 1 (SV40 transcriptional enhancer factor)
  • TEAD1
  • TEAD-1
  • TEF1
  • TEF-1
  • transcription factor 13
  • transcriptional enhancer factor 1
  • transcriptional enhancer factor TEF-1

Background

This gene encodes a ubiquitous transcriptional enhancer factor that is a member of the TEA/ATTS domain family. This protein directs the transactivation of a wide variety of genes and, in placental cells, also acts as a transcriptional repressor. Mutations in this gene cause Sveinsson's chorioretinal atrophy. Additional transcript variants have been described but their full-length natures have not been experimentally verified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TEAD1