TDO2 Antibody (998604) [Alexa Fluor® 700] Summary
Additional Information |
Tryptophan 2,3-dioxygenase |
Immunogen |
E. coli-derived human TDO2 Leu18-Phe388 Accession # P48775 |
Specificity |
Detects human TDO2 in direct ELISAs. |
Source |
N/A |
Isotype |
IgG2b |
Clonality |
Monoclonal |
Host |
Mouse |
Purity Statement |
Protein A or G purified from hybridoma culture supernatant |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Intracellular Staining by Flow Cytometry 0.25-1 ug/10^6 cells
|
Packaging, Storage & Formulations
Storage |
Protect from light. Do not freeze.- 12 months from date of receipt, 2 to 8 °C as supplied.
|
Buffer |
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide. |
Notes
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for TDO2 Antibody (998604) [Alexa Fluor® 700]
Background
Tryptophan 2,3-dioxygenase (TDO2), a heme-containing cytosolic
dioxygenase, forms a homo-tetrameric active molecule of approximately 190 kDa
composed of 48 kDa monomers (1, 2). Human TDO2 shares 89% aa sequence
identity with mouse TDO2. TDO2 is one of three proteins capable
of catalyzing the first and rate‑limiting step of the L-kynurenine pathway (KP):
oxidative cleavage of the essential amino acid L-tryptophan to form N‑formyl‑kynurenine
(3). TDO2 is a cytosolic protein typically localized to the liver and brain, unlike
the more ubiquitously expressed indoleamine 2,3-dioxygenase (IDO), yet it is
responsible for ~90% of the primary route of catabolism of tryptophan through
the KP (3). TDO2 is upregulated in extrahepatic tumors (4-6) and is
consequently a target in cancer immunotherapy (7). TDO2 is a therapeutic target
in brain disease such as schizophrenia, Alzheimers disease, multiple sclerosis
and glioma (8-11) due to its role in the regulation of levels of critical
biologically active downstream KP metabolites (3). Polymorphisms in the TDO2
gene have been implicated for a role in behavioural responses and autism (12,13).
- Lewis-Ballester, A. et al. (2016) Sci. Rep. 6:35169.
- Rafice, S.A. et al. (2009) Biochem. Soc. Trans. 37:408.
- Badawy, A. (2017) Int J. Tryptophan Res. 10:1.
- Pilotte, L. et al. (2012) Proc. Natl. Acad. Sci. U.S.A. 109:2497.
- D'Amato, N.C. et al. (2015) Cancer Res. 75:4651.
- Yu, C.P et al. (2017) Med. Oncol. 34:73.
- Platten, et al. (2015) Front. Immunol. 5:673.
- Breda, C. et al. (2016) Proc. Natl. Acad. Sci. U.S.A. 113:5435.
- Yu, C.P. et al. (2016) Metab. Brain Dis. 31:737.
- Lanz. T.V. et al. (2017) Sci. Rep. 7:41271.
- Reus, G.Z. et al. (2018) Prog. Neuropsychopharmacol. Biol. Psychiatry 81:55.
- Soichot, M. et al. (2013) Alcohol Alcohol 48:415.
- Nabi, R. et al. (2004) Am. J. Med. Genet. B. Neuropsychiatr. Genet. 125B:63.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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