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TDG Antibody

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Genetic Strategies: Knockout Validated: TDG Antibody [NBP2-20627] - Wild-type (WT) and TDG knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TDG antibody. ...read more
Immunocytochemistry/ Immunofluorescence: TDG Antibody [NBP2-20627] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TDG protein stained by TDG antibody diluted at 1:1000. Blue: Hoechst 33342 ...read more
Western Blot: TDG Antibody [NBP2-20627] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TDG antibody (NBP2-20627) diluted at ...read more
Western Blot: TDG Antibody [NBP2-20627] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TDG antibody (NBP2-20627) diluted at 1:500.
Genetic Strategies: Western Blot: TDG Antibody [NBP2-20627] - Wild-type (WT) and TDG knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TDG antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications WB, ICC/IF, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

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TDG Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human TDG. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Bovine (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TDG
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:5000-1:20000
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat (84%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 20% Glycerol (pH7)
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for TDG Antibody

  • EC 3.2.2.29
  • TDG
  • thymine-DNA glycosylaseG/T mismatch-specific thymine DNA glycosylase

Background

The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TDG Antibody (NBP2-20627) (0)

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Secondary Antibodies

 

Isotype Controls

Additional TDG Products

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Bioinformatics

Gene Symbol TDG
Uniprot