![Western Blot: TCF-3/E2A Antibody (HL1954) - Azide and BSA Free [NBP3-25713] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCF3 / E2A antibody [HL1954] (NBP3-25713) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25713_rabbit-tcf-3-e2a-mab-hl1954-azide-and-bsa-free-2120249342773.jpg "Western Blot: TCF-3/E2A Antibody (HL1954) - Azide and BSA Free [NBP3-25713] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCF3 / E2A antibody [HL1954] (NBP3-25713) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF |
| Clone | HL1954 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant fragment of human TCF-3/E2A |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | TCF3 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 68 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for TCF-3/E2A Antibody (NBP3-25713)Find related products by research area.
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| Gene Symbol | TCF3 |
![Western Blot: TCF-3/E2A Antibody (HL1954) - Azide and BSA Free [NBP3-25713] - NCI-H929 whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCF3 / E2A antibody [HL1954] (NBP3-25713) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25713_rabbit-tcf-3-e2a-mab-hl1954-azide-and-bsa-free-2120249374994.jpg "Western Blot: TCF-3/E2A Antibody (HL1954) - Azide and BSA Free [NBP3-25713] - NCI-H929 whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCF3 / E2A antibody [HL1954] (NBP3-25713) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: TCF-3/E2A Antibody (HL1954) - Azide and BSA Free [NBP3-25713] - TCF3 / E2A antibody [HL1954] detects TCF3 / E2A protein by immunofluorescent analysis. Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TCF3 / E2A stained by TCF3 / E2A antibody [HL1954] (NBP3-25713) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-25713_rabbit-tcf-3-e2a-mab-hl1954-azide-and-bsa-free-2120249362720.jpg "Immunocytochemistry/Immunofluorescence: TCF-3/E2A Antibody (HL1954) - Azide and BSA Free [NBP3-25713] - TCF3 / E2A antibody [HL1954] detects TCF3 / E2A protein by immunofluorescent analysis. Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TCF3 / E2A stained by TCF3 / E2A antibody [HL1954] (NBP3-25713) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.")
![Immunocytochemistry/Immunofluorescence: TCF-3/E2A Antibody (HL1954) - Azide and BSA Free [NBP3-25713] - TCF3 / E2A antibody [HL1954] detects TCF3 / E2A protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TCF3 / E2A stained by TCF3 / E2A antibody [HL1954] (NBP3-25713) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp3-25713_rabbit-tcf-3-e2a-mab-hl1954-azide-and-bsa-free-2120249422619.jpg "Immunocytochemistry/Immunofluorescence: TCF-3/E2A Antibody (HL1954) - Azide and BSA Free [NBP3-25713] - TCF3 / E2A antibody [HL1954] detects TCF3 / E2A protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TCF3 / E2A stained by TCF3 / E2A antibody [HL1954] (NBP3-25713) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")