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T-bet/TBX21 Antibody (39D) [Janelia Fluor® 646]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clone
39D
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 646

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-43299AF350
Alexa Fluor 405
NBP1-43299AF405
Alexa Fluor 488
NBP1-43299AF488
Alexa Fluor 532
NBP1-43299AF532
Alexa Fluor 594
NBP1-43299AF594
Alexa Fluor 647
NBP1-43299AF647
Alexa Fluor 700
NBP1-43299AF700
Alexa Fluor 750
NBP1-43299AF750
Biotin
NBP1-43299B
CoraFluor 1
NBP1-43299CL1
DyLight 350
NBP1-43299UV
DyLight 405
NBP1-43299V
DyLight 488
NBP1-43299G
DyLight 550
NBP1-43299R
DyLight 594
NBP1-43299DL594
DyLight 650
NBP1-43299C
DyLight 755
NBP1-43299IR
FITC
NBP1-43299F
HRP
NBP1-43299H
Janelia Fluor 525
NBP1-43299JF525
Janelia Fluor 549
NBP1-43299JF549
Janelia Fluor 669
NBP1-43299JF669
Unconjugated
NBP1-43299-0.1mg
mFluor Violet 450 SE
NBP1-43299MFV450
mFluor Violet 500 SE
NBP1-43299MFV500
mFluor Violet 610 SE
NBP1-43299MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-43299-0.1mg
BSA Free
NBP1-43299-0.1mg
BSA Free
NBP1-43299-0.025mg
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T-bet/TBX21 Antibody (39D) [Janelia Fluor® 646] Summary

Immunogen
The immunogen for this antibody was raised against TBX21.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TBX21
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for T-bet/TBX21 Antibody (39D) [Janelia Fluor® 646]

  • Tbet
  • T-bet
  • TBETT-PET
  • TBLYM
  • TBLYMT-cell-specific T-box transcription factor T-bet
  • T-box 21
  • T-box expressed in T cells
  • T-box protein 21
  • T-box transcription factor TBX21
  • TBX21
  • T-PET
  • Transcription factor TBLYM

Background

Tbx21 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for T-bet/TBX21 Antibody (NBP1-43299JF646). (Showing 1 - 1 of 1 FAQ).

  1. Do you make a Tbx21 ab for FACS for porcine?
    • We have not tested our Tbx21 antibodies for porcine, however you might like to test NBP1-43298 as part of our Innovators Reward Program. This is gained from testing a product on a species or application that we have not yet tested. I have carried out a blast to check the % homology between the pig protein and human protein, this is 94% and our lab recommends use if the % homology is 85% or above.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TBX21