![Western Blot: Syntaxin-BP1 Antibody [NBP2-20525] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Syntaxin-BP1 antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Syntaxin-BP1-Antibody-Western-Blot-NBP2-20525-img0005.jpg "Western Blot: Syntaxin-BP1 Antibody [NBP2-20525] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Syntaxin-BP1 antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Ze, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Syntaxin-BP1. The exact sequence is proprietary. |
| Localization | Cytoplasm; Membrane; Peripheral membrane protein |
| Predicted Species | Rhesus Macaque (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | STXBP1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 68 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: Syntaxin-BP1 Antibody [NBP2-20525] - DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: Syntaxin-BP1 protein stained by Syntaxin-BP1 antibody diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Syntaxin-BP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20525-img0004.jpg "Immunocytochemistry/Immunofluorescence: Syntaxin-BP1 Antibody [NBP2-20525] - DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: Syntaxin-BP1 protein stained by Syntaxin-BP1 antibody diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Paraffin: Syntaxin-BP1 Antibody [NBP2-20525] - U373 xenograft, using STXBP1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/STXBP1-Antibody-Immunohistochemistry-Paraffin-NBP2-20525-img0003.jpg "Immunohistochemistry-Paraffin: Syntaxin-BP1 Antibody [NBP2-20525] - U373 xenograft, using STXBP1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: Syntaxin-BP1 Antibody [NBP2-20525] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/STXBP1-Antibody-Western-Blot-NBP2-20525-img0002.jpg "Western Blot: Syntaxin-BP1 Antibody [NBP2-20525] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:1000.")
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