Does this product work on endometrial tissue?

Name: Syndecan-1/CD138 Antibody (B-A38)
Brand: Novus Biologicals
SKU: NB100-64980
Rating: 5 (1 reviews)

Question

Accepted Answer

Syndecan-1/CD138 antibody (NB100-64980) is a great antibody and it should work in any sections as long as they have CD138 antigen. As far as endometrial tissue is concerned, I am assuming that the cancerous endometrial tissue express Syndecan-1/CD138 while the normal tissue does not express it that much. And we would suggest the customer to include a positive control section in the assay using any of the following tissues: Normal Tissues:- Placenta, Colon, Small Intestine or Tonsil; Cancerous Tissues:- Ovarian cancer, rectal cancer, Lung cancer.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	WB, Flow, Func, IHC
Clone	B-A38
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated
Concentration	0.1 mg/ml

Additional Information	Clone B-A38 was used by HLDA to establish CD designation.
Immunogen	U266 cell line
Localization	Membrane; Single-pass type I membrane protein.
Specificity	NB100-64980 recognizes a heparan sulfate rich membrane glycoprotein of 200kD known as Syndecan-1 (CD138). Syndecan functions as a receptor for collagen, fibronectin and thrombospondin. NB100-64980 stains all plasma cells and plasma cell lines. No staining of other leucocytes is seen in peripheral blood or bone marrow, although some ALL cell lines express the antigen weakly. Some staining of epithelial and endothelial cells may be seen in immunohistology.
Isotype	IgG1
Clonality	Monoclonal
Host	Mouse
Gene	SDC1
Purity	Ion exchange chromatography
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	PBS
Preservative	0.09% Sodium Azide
Concentration	0.1 mg/ml
Purity	Ion exchange chromatography

Publications using NB100-64980	Applications	Species
Lin SC, Wu CP, Tseng T, Jhang Y et al. Role of syndecan-1 and exogenous heparin in hepatoma sphere formation Biochem. Cell Biol. 2019-05-01 [PMID: 31042409]
Sharpe B, Alghezi DA, Cattermole C et al. A subset of high Gleason grade prostate carcinomas contain a large burden of prostate cancer syndecan-1 positive stromal cells Prostate 2017-07-26 [PMID: 28744948] (ICC/IF, Human)	ICC/IF	Human
Wang C, Tseng T, Jhang Y et al. Loss of cell invasiveness through PKC-mediated syndecan-1 downregulation in melanoma cells under anchorage-independency. Exp Dermatol. 2014-09-18 [PMID: 25236603]
Li, K et al. Anaplastic lymphoma kinase-positive diffuse large B-cell lymphoma presenting as an isolated nasopharyngeal mass: a case report and review of literature. Int J Clin Exp Pathol 4: 190-6. 2010-01-01 [PMID: 21326807]
Beauvais, DM Rapraeger, AC. Syndecan-1 couples the insulin-like growth factor-1 receptor to inside-out integrin activation. J Cell Sci 123: 3796-807. 2010-01-01 [PMID: 20971705]
Thaunat, O et al. Chronic rejection triggers the development of an aggressive intragraft immune response through recapitulation of lymphoid organogenesis. J Immunol 185: 717-28. 2010-01-01 [PMID: 20525884]
Du, S et al. Systemic mastocytosis in association with chronic lymphocytic leukemia and plasma cell myeloma. Int J Clin Exp Pathol 3: 448-57. 2010-01-01 [PMID: 20490336]
Chang, H et al. CKS1B nuclear expression is inversely correlated with p27Kip1 expression and is predictive of an adverse survival in patients with multiple myeloma. 20421271 Haematologica. 95: 1542-7. 2010-01-01 [PMID: 20421271]
Kim, YC et al. Presence of Porphyromonas gingivalis and plasma cell dominance in gingival tissues with periodontitis. Oral Dis 16: 375-81. 2010-01-01 [PMID: 20233323]
Kylanpaa, L et al. Syndecan-1 and tenascin expression in cystic tumors of the pancreas. JOP 10: 378-82. 2009-01-01 [PMID: 19581738]
Beauvais, DM et al. Syndecan-1 regulates alphavbeta3 and alphavbeta5 integrin activation during angiogenesis and is blocked by synstatin, a novel peptide inhibitor. J Exp Med 206: 691-705. 2009-01-01 [PMID: 19255147]
Mahshid, Y et al. High expression of 5-lipoxygenase in normal and malignant mantle zone B lymphocytes. BMC Immunol;10:2. 2009-01-09 [PMID: 19134178]
Yang, Y et al. The syndecan-1 heparan sulfate proteoglycan is a viable target for myeloma therapy. Blood 110: 2041-8. 2007-01-01 [PMID: 17536013]
Guedez, L et al. Tissue inhibitor of metalloproteinase 1 (TIMP-1) promotes plasmablastic differentiation of a Burkitt lymphoma cell line: implications in the pathogenesis of plasmacytic/plasmablastic tumors. Blood 105: 1660-8. 2005-01-01 [PMID: 15479729]
Anttonen, A et al. (1999) Syndecan-1 expression has prognostic significance in head and neck carcinoma. Brit J Cancer 79: 558 - 564. 1999-01-01 [PMID: 10027330]
Borset, M et al. (1993) Lack of IL-1 secretion from human myeloma cells highly purified by immunomagnetic separation. Br J Haem 85: 446. 1993-01-01 [PMID: 8136264]
Angelioudaki I, Kataki A, Koniaris E, Tzingounis AG INTERCONNECTION OF EPITHELIAL-MESENCHYMAL TRANSITION AND SENESCENCE IN PANCREATIC NEUROENDOCRINE LESIONS Thesis 2022-01-01
Show All 17 Publications. Collapse Publications.

Syndecan-1/CD138 Antibody (B-A38)

Species Glossary

Images

Product Details

Order Details

Syndecan-1/CD138 Antibody (B-A38) Summary

Applications/Dilutions

Packaging, Storage & Formulations

Alternate Names for Syndecan-1/CD138 Antibody (B-A38)

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for Syndecan-1/CD138 Antibody (NB100-64980)(17)

Review for Syndecan-1/CD138 Antibody (NB100-64980) (1) 51

Summary

Comments

Product General Protocols

Video Protocols

FAQs for Syndecan-1/CD138 Antibody (NB100-64980). (Showing 1 - 2 of 2 FAQs).

Secondary Antibodies

Isotype Controls

Additional Syndecan-1/CD138 Products

Blogs on Syndecan-1/CD138

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Recent Reviews

Bioinformatics

Gene Symbol	SDC1
Entrez	6382(Human)
Uniprot	P18827(Human)

	Verified Customer 09/14/2023
Application:	IHC-P
Species:	Human