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Synaptophysin Antibody (7H12) - BSA Free

Images

 
Western Blot: Synaptophysin Antibody (7H12) [NBP1-47483] - Analysis using Synaptophysin mouse mAb against rat brain tissue lysate.
Immunohistochemistry-Paraffin: Synaptophysin Antibody (7H12) [NBP1-47483] - Human pancreas (FFPE) stained with Synaptophysin antibody. pH 9 HIER antigen retrieval. Image from verified customer review.
Immunohistochemistry-Paraffin: Synaptophysin Antibody (7H12) [NBP1-47483] - Analysis of paraffin-embedded human small intestine, myenteric plexus tissues using anti-Synaptophysin mouse mAb.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, IHC
Clone
7H12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

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Synaptophysin Antibody (7H12) - BSA Free Summary

Immunogen
Purified recombinant fragment of human Synaptophysin expressed in E. coli. [UniProt# P08247]
Marker
Pre-synaptic marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SYP
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
Theoretical MW
33.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-47483 in the following applications:

Publications
Read Publications using
NBP1-47483 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Sodium Azide
Concentration
1.0 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for Synaptophysin Antibody (7H12) - BSA Free

  • Major synaptic vesicle protein p38
  • MRX
  • MRXSYP
  • Synaptophysin
  • SYP

Background

Synaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells.Synaptophysin contains four transmembrane domains that form a hexameric channel or gap junction-like pore. Synaptophysin binds to the SNARE protein synaptobrevin/VAMP, which prevents the inclusion of synaptobrevin in the synaptic vesicle fusion complex and creates a pool of synaptobrevin for exocytosis when synapse activity increases. Synaptophysin is also responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, a critical component of the fusion complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Synaptophysin Antibody (NBP1-47483)(3)

Review for Synaptophysin Antibody (NBP1-47483) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-47483:
Filter by Applications
Immunofluorescence - paraffin
(1)
All Applications
Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Other Synaptophysin NBP1-47483
Enlarge
5
reviewed by:
Verified Customer
Immunofluorescence - paraffin Human 01/20/2019
View

Summary

ApplicationOther
Sample TestedAdult pancreas
SpeciesHuman
LotI don't have it any more

Comments

CommentspH 9 antigen retrieval

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Synaptophysin Antibody (NBP1-47483) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Synaptophysin Products

Research Areas for Synaptophysin Antibody (NBP1-47483)

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Blogs on Synaptophysin.

Losing memory: Toxicity from mutant APP and amyloid beta explain the hippocampal neuronal damage in Alzheimer's disease
 By Jamshed Arslan Pharm.D.  Alzheimer's disease (AD) is an irreversible brain disorder that destroys memory and thinking skills. The telltale signs of AD brains are extracellular deposits of amy...  Read full blog post.

Synaptophysin a Marker Protein in Neuroendocrine Cells
Synaptophysin a Marker Protein in Neuroendocrine Cells Synaptophysin is a major integral membrane glycoprotein of neuronal synaptic vesicles present in virtually all synapses and shows a high degree of evolutionary conservation across the mammals. Syn...  Read full blog post.

Characterizing Synaptophysin is "a Snap"
Synaptophysin is an integral membrane glycoprotein found within the small synaptic vesicles in brain and endocrine cells. Studies with synaptophysin antibodies show that it is one of the most abundant small vesicle proteins, constituting approximately...  Read full blog post.

Synaptophysin and Dementing Disorders
 Synaptophysin (a presynaptic vesicle protein) is an integral membrane glycoprotein originally isolated from presynaptic vesicles of bovine neurons. Synaptophysin is found in all nerve terminals and synaptophysin measurements have been used to quantif...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Verified Customer
01/20/2019
Application: Immunofluorescence - paraffin
Species: Human

Bioinformatics

Gene Symbol SYP
Entrez
Uniprot