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STK38 Antibody (3A5)

Images

 
Western Blot: STK38 Antibody (3A5) [H00011329-M02] - STK38 monoclonal antibody (M02), clone 3A5. Analysis of STK38 expression in Raw 264.7.
Immunohistochemistry-Paraffin: STK38 Antibody (3A5) [H00011329-M02] - Analysis of monoclonal antibody to STK38 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 1 ug/ml.
Western Blot: STK38 Antibody (3A5) [H00011329-M02] - STK38 monoclonal antibody (M02), clone 3A5. Analysis of STK38 expression in PC-12.
Western Blot: STK38 Antibody (3A5) [H00011329-M02] - STK38 monoclonal antibody (M02), clone 3A5 Analysis of STK38 expression in Hela S3 NE.
Western Blot: STK38 Antibody (3A5) [H00011329-M02] - STK38 monoclonal antibody (M02), clone 3A5. Analysis of STK38 expression in NIH/3T3.
ELISA: STK38 Antibody (3A5) [H00011329-M02] - Detection limit for recombinant GST tagged STK38 is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC
Clone
3A5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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STK38 Antibody (3A5) Summary

Immunogen
STK38 (AAH12085, 365 a.a. ~ 465 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EHRIGAPGVEEIKSNSFFEGVDWEHIRERPAAISIEIKSIDDTSNFDEFPESDILKPTVATSNHPETDYKNKDWVFINYTYKRFEGLTARGAIPSYMKAAK
Localization
Cytoplasmic and Nuclear
Specificity
STK38 - serine/threonine kinase 38
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
STK38
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Publications
Read Publication using
H00011329-M02 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for STK38 Antibody (3A5)

  • EC 2.7.11
  • EC 2.7.11.1
  • Ndr Ser/Thr kinase-like protein
  • NDR1 protein kinase
  • NDR1
  • NDRnuclear Dbf2-related 1
  • Nuclear Dbf2-related kinase 1
  • serine threonine protein kinase
  • serine/threonine kinase 38
  • serine/threonine-protein kinase 38

Background

STK38 belongs to the NDR family of serine/threonine protein kinases. NDR kinases require the phosphorylation of conserved Ser/Thr residues for activation. NDR family members have two unique stretches of primary sequence: an N-terminal regulatory (NTR) domain and an insert of several residues between subdomains VII and VIII of the kinase domain. The kinase domain insert functions as an auto-inhibitory sequence (AIS), while binding of the co-activator MOB (Mps-one binder) proteins to the NTR domain releases NDR kinases from inhibition of autophosphorylation. STK38 negatively regulates the activation of MEKK1/2 by direct interaction with the catalytic domain of MEKK1/2. The negative regulation of MEKK1/2 is not due to its phosphorylation by STK38.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for STK38 Antibody (H00011329-M02)(1)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol STK38
Entrez
OMIM
Uniprot