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STEAP1 Antibody (4F6-1F3)

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Western Blot: STEAP1 Antibody (4F6-1F3) [H00026872-M01] - STEAP1 monoclonal antibody (M01), clone 4F6-1F3 Analysis of STEAP1 expression in A-431.
Immunocytochemistry/ Immunofluorescence: STEAP1 Antibody (4F6-1F3) [H00026872-M01] - Analysis of monoclonal antibody to STEAP1 on A-431 cell. Antibody concentration 10 ug/ml.
Western Blot: STEAP1 Antibody (4F6-1F3) [H00026872-M01] - Analysis of STEAP1 expression in transfected 293T cell line by STEAP1 monoclonal antibody (M01), clone 4F6-1F3.Lane 1: STEAP1 transfected lysate (Predicted MW: ...read more
ELISA: STEAP1 Antibody (4F6-1F3) [H00026872-M01] - Detection limit for recombinant GST tagged STEAP1 is approximately 0.3ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
4F6-1F3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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STEAP1 Antibody (4F6-1F3) Summary

Immunogen
STEAP1 (AAH11802, 1 a.a. ~ 339 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MESRKDITNQEELWKMKPRRNLEEDDYLHKDTGETSMLKRPVLLHLHQTAHADEFDCPSELQHTQELFPQWHLPIKIAAIIASLTFLYTLLREVIHPLATSHQQYFYKIPILVINKVLPMVSITLLALVYLPGVIAAIVQLHNGTKYKKFPHWLDKWMLTRKQFGLLSFFFAVLHAIYSLSYPMRRSYRYKLLNWAYQQVQQNKEDAWIEHDVWRMEIYVSLGIVGLAILALLAVTSIPSVSDSLTWREFHYIQSKLGIVSLLLGTIHALIFAWNKWIDIKQFVWYTPPTFMIAVFLPIVVLIFKSILFLPCLRKKILKIRHGWEDVTKINKTEICSQL
Specificity
STEAP1 - six transmembrane epithelial antigen of the prostate 1 (4F6-1F3)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
STEAP1
Purity
Ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1:500

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
Ascites

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for STEAP1 Antibody (4F6-1F3)

  • EC 1.16.1.-
  • PRSS24
  • PRSS24MGC19484
  • six transmembrane epithelial antigen of the prostate 1
  • six transmembrane epithelial antigen of the prostate
  • Six-transmembrane epithelial antigen of prostate 1
  • STEAP1
  • STEAPmetalloreductase STEAP1

Background

This gene is predominantly expressed in prostate tissue, and is found to be upregulated in multiple cancer cell lines. The gene product is predicted to be a six-transmembrane protein, and was shown to be a cell surface antigen significantly expressed at cell-cell junctions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for STEAP1 Antibody (H00026872-M01). (Showing 1 - 1 of 1 FAQ).

  1. May any of your anti human-STEAP1 Abs be used for flow cytometry, with a secondary anti-mouse antibody? E.g. J2D2 or 4F6-1F3?
    • Unfortunately, none of our STEAP1 antibodies have been validated in flow cytometry yet. This does not mean they will not work in FLOW, we just cannot guarantee its use in FLOW application because we have not tested them in this application in our lab yet. However, if you would like to try one of these antibodies in FLOW, you would be eligible for our Innovator's Reward program, whereby if you share your results with us, we will reward you with a voucher for 100% of the purchase price. Technically, STEAP1 (J2D2) NBP1-07094 and STEAP1 (4F6-1F3) H00026872-M01 are the two antibodies that have more chances of success because of proven compatibility with IF labeled secondary antibodies as they have already been validated in ICC-IF.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol STEAP1
OMIM