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Human SRD5A2 - Ready-To-Use ELISA Kit (Colorimetric)

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ELISA: Human SRD5A2 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40123] - Standard Curve Reference
ELISA: Human SRD5A2 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40123] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Standard Curve Range
0.312 - 20 ng/mL (example only; lot dependent)
Sensitivity
0.126 ng/mL (example only; lot dependent)

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Human SRD5A2 - Ready-To-Use ELISA Kit (Colorimetric) Summary

Description
The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours
Standard Curve Range
0.312 - 20 ng/mL (example only; lot dependent)
Sensitivity
0.126 ng/mL (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12 (example only; lot dependent)
Intra-Assay
%CV < 10 (example only; lot dependent)
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
SRD5A2

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Detection Solution A
  2. Detection Solution B
  3. Instruction manual
  4. Plate sealer for 96 wells
  5. Pre-coated 96T strip plate
  6. Standard Diluent
  7. Standard
  8. Stop Solution
  9. TMB Substrate
  10. Wash Buffer (30 x concentrate)

Alternate Names for Human SRD5A2 - Ready-To-Use ELISA Kit (Colorimetric)

  • 3-oxo-5-alpha-steroid 4-dehydrogenase 2,5 alpha-SR2
  • 5 alpha-SR2
  • EC 1.3.99.5
  • MGC138457
  • S5AR 2
  • SR type 2
  • SRD5A2
  • Steroid 5-alpha-reductase 2,3-oxo-5 alpha-steroid 4-dehydrogenase 2
  • steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta4-dehydrogenase alpha 2)
  • Type II 5-alpha reductase

Background

SRD5A2 encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol SRD5A2