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SRD5A2 Antibody

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Western Blot: SRD5A2 Antibody [NB300-981] - Staining of Human Prostate lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: SRD5A2 Antibody [NB300-981] - Staining of Human Prostate lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Hamster
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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SRD5A2 Antibody Summary

Immunogen
Peptide with sequence QVQCQQSPVLAG corresponding to N-Terminus according to NP_000339.2.
Isotype
IgG
Clonality
Polyclonal
Host
Hamster
Gene
SRD5A2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.3-1 ug/ml
Application Notes
WB: Approx 26kDa band observed in Human Prostate and Breast lysates (calculated MW of 28.4kDa according to NP_000339.2).
Publications
Read Publication using NB300-981.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SRD5A2 Antibody

  • 3-oxo-5-alpha-steroid 4-dehydrogenase 2,5 alpha-SR2
  • 5 alpha-SR2
  • EC 1.3.99.5
  • MGC138457
  • S5AR 2
  • SR type 2
  • SRD5A2
  • Steroid 5-alpha-reductase 2,3-oxo-5 alpha-steroid 4-dehydrogenase 2
  • steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta4-dehydrogenase alpha 2)
  • Type II 5-alpha reductase

Background

This gene encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SRD5A2 Antibody (NB300-981)(1)


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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SRD5A2
Entrez
Uniprot