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SPFH2 Antibody

Images

 
Western Blot: SPFH2 Antibody [NBP1-31479] - Sample(30 ug of whole cell lysate)A:HeLa S3 12% SDS PAGE, antibody diluted at 1:500.
Immunohistochemistry-Paraffin: SPFH2 Antibody [NBP1-31479] - Paraffin-embedded endo mitral OVCA, using antibody at 1:100 dilution.
Western Blot: SPFH2 Antibody [NBP1-31479] - Analysis of NBP1-31479 on HeLa S3, with 12% SDS PAGE, diluted at 1:500.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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SPFH2 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SPFH2. The exact sequence is proprietary.
Localization
Endoplasmic reticulum membrane, Single-pass type II membrane protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ERLIN2
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%) and Rhesus Macaque.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for SPFH2 Antibody

  • C8orf2SPFH domain-containing protein 2
  • Endoplasmic reticulum lipid raft-associated protein 2
  • ER lipid raft associated 2
  • Erlin-2
  • MGC87072
  • NET32
  • SPFH domain family, member 2
  • SPFH2chromosome 8 open reading frame 2
  • Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2

Background

SPFH2 is a component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs). Also involved in ITPR1 degradation by the ERAD pathway

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ERLIN2