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Spermine synthase Antibody (OTI3C9) - Azide and BSA Free

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Western Blot: Spermine synthase Antibody (OTI3C9) - Azide and BSA Free [NBP2-74318] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Spermine synthase (Right lane) cDNA for 48 hrs ...read more
Immunohistochemistry: Spermine synthase Antibody (OTI3C9) - Azide and BSA Free [NBP2-74318] - Staining of paraffin-embedded Human lymphoma tissue using anti-Spermine synthase mouse monoclonal antibody.
Flow Cytometry: Spermine synthase Antibody (OTI3C9) - Azide and BSA Free [NBP2-74318] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by ...read more
Western Blot: Spermine synthase Antibody (OTI3C9) - Azide and BSA Free [NBP2-74318] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Spermine synthase monoclonal antibody.
Immunohistochemistry: Spermine synthase Antibody (OTI3C9) - Azide and BSA Free [NBP2-74318] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Spermine synthase mouse monoclonal antibody.
Immunohistochemistry: Spermine synthase Antibody (OTI3C9) - Azide and BSA Free [NBP2-74318] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Spermine synthase mouse monoclonal ...read more
Immunohistochemistry: Spermine synthase Antibody (OTI3C9) - Azide and BSA Free [NBP2-74318] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Spermine synthase mouse monoclonal antibody.
Immunohistochemistry: Spermine synthase Antibody (OTI3C9) - Azide and BSA Free [NBP2-74318] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Spermine synthase mouse monoclonal antibody.
Immunohistochemistry: Spermine synthase Antibody (OTI3C9) - Azide and BSA Free [NBP2-74318] - Staining of paraffin-embedded Human Kidney tissue using anti-Spermine synthase mouse monoclonal antibody.
Immunohistochemistry: Spermine synthase Antibody (OTI3C9) - Azide and BSA Free [NBP2-74318] - Staining of paraffin-embedded Human tonsil using anti-Spermine synthase mouse monoclonal antibody.
Flow Cytometry: Spermine synthase Antibody (OTI3C9) - Azide and BSA Free [NBP2-74318] - Analysis of Hela cells, using anti-Spermine synthase antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI3C9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

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Spermine synthase Antibody (OTI3C9) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human SMS(NP_004586) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SMS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
Theoretical MW
41.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Spermine synthase Antibody (OTI3C9) - Azide and BSA Free

  • EC 2.5.1.22
  • MRSRSpS
  • Snyder-Robinson X-linked mental retardation syndrome
  • Spermidine aminopropyltransferase
  • spermine synthase
  • SPMSYSRS

Background

Spermine synthase is 1 of 4 enzymes involved in the synthesis of polyamines from arginine and methionine. The reaction catalysed is S-adenosylmethioninamine + spermidine = methylthioadenosine + spermine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SMS