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Spermine synthase Antibody (OTI3C9)

Images

 
Western Blot: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Spermine synthase (Right lane) cDNA for 48 hrs and lysed. ...read more
Immunohistochemistry-Paraffin: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - Staining of paraffin-embedded Human tonsil using anti-Spermine synthase mouse monoclonal antibody.
Flow Cytometry: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Spermine synthase ...read more
Western Blot: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Spermine synthase monoclonal antibody.
Immunohistochemistry-Paraffin: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Spermine synthase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Spermine synthase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Spermine synthase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Spermine synthase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - Staining of paraffin-embedded Human Kidney tissue using anti-Spermine synthase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - Staining of paraffin-embedded Human lymphoma tissue using anti-Spermine synthase mouse monoclonal antibody.
Flow Cytometry: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - Analysis of Hela cells, using anti-Spermine synthase antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI3C9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.92 mg/ml

Order Details

Spermine synthase Antibody (OTI3C9) Summary

Immunogen
Full length human recombinant protein of human SMS(NP_004586) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SMS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
41.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.92 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Spermine synthase Antibody (OTI3C9)

  • EC 2.5.1.22
  • MRSRSpS
  • Snyder-Robinson X-linked mental retardation syndrome
  • Spermidine aminopropyltransferase
  • spermine synthase
  • SPMSYSRS

Background

The protein encoded by this gene belongs to the spermidine/spermine synthases family. This gene encodes an ubiquitous enzyme of polyamine metabolism. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SMS
Entrez