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Recombinant Human SOD2/Mn-SOD His Protein

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SDS-Page: Recombinant Human SOD2/Mn-SOD Protein [NBP1-41138] - Superoxide Dismutase 2 Protein SOD2, 24.4kDa (219aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE
SDS-Page: Recombinant Human SOD2/Mn-SOD Protein [NBP1-41138] - Superoxide Dismutase 2 Protein SOD2, 24.4kDa (219aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, Enzyme Activity
Concentration
0.5 mg/ml

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Recombinant Human SOD2/Mn-SOD His Protein Summary

Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 25-222 of Human SOD2/Mn-SOD

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MKHSLPDLPY DYGALEPHIN AQIMQLHHSK HHAAYVNNLN VTEEKYQEAL AKGDVTAQIA LQPALKFNGG GHINHSIFWT NLSPNGGGEP KGELLEAIKR DFGSFDKFKE KLTAASVGVQ GSGWGWLGFN KERGHLQIAA CPNQDPLQGT TGLIPLLGID VWEHAYYLQY KNVRPDYLKA IWNVINWENV TERYMACKK
Details of Functionality
Specific activity is > 1,000 units/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.5 at 25C.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
SOD2
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • Enzyme Activity
  • SDS-Page
Theoretical MW
24.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH8.0), 20% glycerol
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>95%, by SDS-PAGE

Alternate Names for Recombinant Human SOD2/Mn-SOD His Protein

  • EC 1.15.1.1
  • indophenoloxidase B
  • IPOB
  • IPO-B
  • manganese-containing superoxide dismutase
  • mangano-superoxide dismutase
  • Mn SOD
  • Mn superoxide dismutase
  • MnSOD
  • MVCD6
  • SOD, Mitochodrial
  • SOD2
  • superoxide dismutase [Mn], mitochondrial
  • superoxide dismutase 2, mitochondrial

Background

Superoxide Dismutase 2 is a member of the iron/manganese superoxide dismutase family. It is a mitochondrial matrix protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this protein have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Recombinant human Superoxide Dismutase 2 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol SOD2