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SNAP25 Antibody (OTI4G5) [Janelia Fluor® 549]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI4G5
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 549

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SNAP25 Antibody (OTI4G5) [Janelia Fluor® 549] Summary

Immunogen
Full length recombinant protein of human SNAP25 (NP_003072) produced in HEK293T cells.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
SNAP25
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for SNAP25 Antibody (OTI4G5) [Janelia Fluor® 549]

  • bA416N4.2
  • dJ1068F16.2
  • FLJ23079
  • resistance to inhibitors of cholinesterase 4 homolog
  • RIC4
  • RIC-4
  • SEC9
  • SNAP25
  • SNAP-25SNAP
  • SUP
  • Super protein
  • Synaptosomal-associated 25 kDa protein
  • synaptosomal-associated protein 25
  • synaptosomal-associated protein, 25kD
  • synaptosomal-associated protein, 25kDa

Background

SNAP25 (Synaptosomal associated protein of 25 kDa) is a presynaptic plasma membrane protein that is widely distributed throughout the brain and involved in the regulation of neurotransmitter release. Decreased levels of SNAP25 have been found in the brains of patients with Down Syndrome and Alzheimers Disease (Greber et al.,1999). In addition, a significant reduction in the hippocampal expression of SNAP25 has also been found in patients with Schizophrenia (Fatemi et al., 2001).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SNAP25 Antibody (NBP2-74245JF549) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for SNAP25 Antibody (NBP2-74245JF549)

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Blogs on SNAP25.

Characterizing Synaptophysin is "a Snap"
Synaptophysin is an integral membrane glycoprotein found within the small synaptic vesicles in brain and endocrine cells. Studies with synaptophysin antibodies show that it is one of the most abundant small vesicle proteins, constituting approximately...  Read full blog post.

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Bioinformatics

Gene Symbol SNAP25