![Western Blot: SNAP25 Antibody (4E11) [NBP1-04344] - The extracts of mouse brain (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human SNAP25 (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4E11-Western-Blot-NBP1-04344-img0002.jpg "Western Blot: SNAP25 Antibody (4E11) [NBP1-04344] - The extracts of mouse brain (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human SNAP25 (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF |
| Clone | 4E11 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | Recombinant human SNAP25 (1-206aa) purified from E. coli |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SNAP25 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4), 10% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: SNAP25 Antibody (4E11) [NBP1-04344] - Analysis of SNAP25 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SNAP25 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/SNAP25-Antibody-4E11-Immunocytochemistry-Immunofluorescence-NBP1-04344-img0009.jpg "Immunocytochemistry/Immunofluorescence: SNAP25 Antibody (4E11) [NBP1-04344] - Analysis of SNAP25 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SNAP25 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).")
![Flow Cytometry: SNAP25 Antibody (4E11) [NBP1-04344] - Analysis of SNAP25 in U87MG cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/SNAP25-Antibody-4E11-Flow-Cytometry-NBP1-04344-img0006.jpg "Flow Cytometry: SNAP25 Antibody (4E11) [NBP1-04344] - Analysis of SNAP25 in U87MG cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).")
![Western Blot: SNAP25 Antibody (4E11) [NBP1-04344] - The Cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SNAP25 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: SNAP25 Transfected 293T cell lysate](http://images.novusbio.com/fullsize/SNAP25-Antibody-4E11-Western-Blot-NBP1-04344-img0005.jpg "Western Blot: SNAP25 Antibody (4E11) [NBP1-04344] - The Cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SNAP25 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: SNAP25 Transfected 293T cell lysate")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")