![Western Blot: SLC7A5/LAT1 Antibody (HL2353) - Azide and BSA Free [NBP3-25549] - Boiled and unboiled HeLa whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LAT1 / SLC7A5 antibody [HL2353] (NBP3-25549) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)](http://images.novusbio.com/fullsize/nbp3-25549_rabbit-slc7a5-lat1-mab-hl2353-azide-and-bsa-free-2120249374957.jpg "Western Blot: SLC7A5/LAT1 Antibody (HL2353) - Azide and BSA Free [NBP3-25549] - Boiled and unboiled HeLa whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LAT1 / SLC7A5 antibody [HL2353] (NBP3-25549) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | HL2353 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human SLC7A5/LAT1. The exact sequence is proprietary. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | SLC7A5 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 55 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
![Western Blot NTAL Antibody [Unconjugated]](https://images.novusbio.com/images2/NTAL_AF4066_Western_Blot_5396.jpg)
![Intracellular Staining by Flow Cytometry NTAL Antibody [Unconjugated]](https://images.novusbio.com/images2/NTAL_AF4066_Flow_Cytometry_8223.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for SLC7A5/LAT1 Antibody (NBP3-25549)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SLC7A5 |
![Immunohistochemistry-Paraffin: SLC7A5/LAT1 Antibody (HL2353) - Azide and BSA Free [NBP3-25549] - LAT1 / SLC7A5 antibody [HL2353] detects LAT1 / SLC7A5 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. LAT1 / SLC7A5 stained by LAT1 / SLC7A5 antibody [HL2353] (NBP3-25549) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25549_rabbit-slc7a5-lat1-mab-hl2353-azide-and-bsa-free-212024940887.jpg "Immunohistochemistry-Paraffin: SLC7A5/LAT1 Antibody (HL2353) - Azide and BSA Free [NBP3-25549] - LAT1 / SLC7A5 antibody [HL2353] detects LAT1 / SLC7A5 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. LAT1 / SLC7A5 stained by LAT1 / SLC7A5 antibody [HL2353] (NBP3-25549) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: SLC7A5/LAT1 Antibody (HL2353) - Azide and BSA Free [NBP3-25549] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LAT1 / SLC7A5 antibody [HL2353] (NBP3-25549) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25549_rabbit-slc7a5-lat1-mab-hl2353-azide-and-bsa-free-2120249403898.jpg "Western Blot: SLC7A5/LAT1 Antibody (HL2353) - Azide and BSA Free [NBP3-25549] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LAT1 / SLC7A5 antibody [HL2353] (NBP3-25549) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Knockdown Validated: SLC7A5/LAT1 Antibody (HL2353) - Azide and BSA Free [NBP3-25549] - Non-transfected (-) and transfected (+) MCF-7 whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LAT1 / SLC7A5 antibody [HL2353] (NBP3-25549) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25549_rabbit-slc7a5-lat1-mab-hl2353-azide-and-bsa-free-2120249403879.jpg "Knockdown Validated: SLC7A5/LAT1 Antibody (HL2353) - Azide and BSA Free [NBP3-25549] - Non-transfected (-) and transfected (+) MCF-7 whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LAT1 / SLC7A5 antibody [HL2353] (NBP3-25549) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: SLC7A5/LAT1 Antibody (HL2353) - Azide and BSA Free [NBP3-25549] - LAT1 / SLC7A5 antibody [HL2353] detects LAT1 / SLC7A5 protein at cell membrane by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: LAT1 / SLC7A5 stained by LAT1 / SLC7A5 antibody [HL2353] (NBP3-25549) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25549_rabbit-slc7a5-lat1-mab-hl2353-azide-and-bsa-free-21202493915.jpg "Immunocytochemistry/Immunofluorescence: SLC7A5/LAT1 Antibody (HL2353) - Azide and BSA Free [NBP3-25549] - LAT1 / SLC7A5 antibody [HL2353] detects LAT1 / SLC7A5 protein at cell membrane by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: LAT1 / SLC7A5 stained by LAT1 / SLC7A5 antibody [HL2353] (NBP3-25549) diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Western Blot: SLC7A5/LAT1 Antibody (HL2353) - Azide and BSA Free [NBP3-25549] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LAT1 / SLC7A5 antibody [HL2353] (NBP3-25549) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25549_rabbit-slc7a5-lat1-mab-hl2353-azide-and-bsa-free-2120249415938.jpg "Western Blot: SLC7A5/LAT1 Antibody (HL2353) - Azide and BSA Free [NBP3-25549] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LAT1 / SLC7A5 antibody [HL2353] (NBP3-25549) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Bioactivity CTLA-4 [Unconjugated]](https://images.novusbio.com/images2/CTLA4_7268CT_2293.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_871.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_872.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/VEGF_DVE00_ELISA_208.jpg)
![N/A BDNF [HRP]](https://images.novusbio.com/images2/DATA_BDNF_DBD00_ELISA_587.jpg)
![N/A BDNF [HRP]](https://images.novusbio.com/images2/DATA_BDNF_DBD00_ELISA_588.jpg)
![N/A BDNF [HRP]](https://images.novusbio.com/images2/BDNF_DBD00_ELISA_36.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")