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SLC39A5 Antibody

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Western Blot: SLC39A5 Antibody [NBP1-69296] - This Anti-SLC39A5 antibody was used in Western Blot of HepG2 tissue lysate at a concentration of 0.5ug/ml.
Immunohistochemistry: SLC39A5 Antibody [NBP1-69296] - Human Liver.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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SLC39A5 Antibody Summary

Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Immunogen
Synthetic peptides corresponding to SLC39A5(solute carrier family 39 (metal ion transporter), member 5) The peptide sequence was selected from the N terminal of SLC39A5. Peptide sequence MGSPVSHLLAGFCVWVVLGWVGGSVPNLGPAEQEQNHYLAQLFGLYGENG. The peptide sequence for this immunogen was taken from within the described region.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC39A5
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1.0 ug/ml
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for SLC39A5 Antibody

  • LZT-Hs7
  • MGC34778
  • solute carrier family 39 (metal ion transporter), member 5
  • Solute carrier family 39 member 5
  • zinc transporter ZIP5
  • ZIP5
  • ZIP-5
  • Zrt- and Irt-like protein 5

Background

Zinc is an essential cofactor for hundreds of enzymes. It is involved in protein, nucleic acid, carbohydrate, and lipid metabolism, as well as in the control of gene transcription, growth, development, and differentiation. SLC39A5 belongs to a subfamily of proteins that show structural characteristics of zinc transporters.Zinc is an essential cofactor for hundreds of enzymes. It is involved in protein, nucleic acid, carbohydrate, and lipid metabolism, as well as in the control of gene transcription, growth, development, and differentiation. SLC39A5 belongs to a subfamily of proteins that show structural characteristics of zinc transporters (Taylor and Nicholson, 2003).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

FAQs for SLC39A5 Antibody (NBP1-69296). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in antibody NBP1-69296, and I see two bands in the WB result in the datasheet at around 56kDa and another band at around 42kDa. I've checked in uniprot, http://www.uniprot.org/uniprot/Q6ZMH5 Uniprot , and found no isoforms of this protein.
    • From Uniprot, this protein has an expected molecular weight of 56kDa. So I would expect to see this band in samples where the full protein is present. This protein does also have a signal peptide. I would assume that the smaller band (42kDa) is the cleaved protein (without the signal peptide). Depending on the samples, this band may or may not be present. You may need to research the literature to see how to induce this cleavage or what tissues are suppose to express the cleaved protein to be sure.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SLC39A5
Uniprot