Novus Biologicals products are now on bio-techne.com

Sin1/MAPKAP1 Antibody

Images

 
Western Blot: Sin1/MAPKAP1 Antibody [NBP1-89569] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: Sin1/MAPKAP1 Antibody [NBP1-89569] - Staining of human testis shows high expression.
Immunohistochemistry-Paraffin: Sin1/MAPKAP1 Antibody [NBP1-89569] - Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.
Immunohistochemistry-Paraffin: Sin1/MAPKAP1 Antibody [NBP1-89569] - Staining of human pancreas shows low expression as expected.
Orthogonal Strategies: Immunohistochemistry-Paraffin: Sin1/MAPKAP1 Antibody [NBP1-89569] - Staining in human testis and pancreas tissues using anti-MAPKAP1 antibody. Corresponding MAPKAP1 RNA-seq data are ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Sin1/MAPKAP1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: NPFNEYSKFDGKGHVGTTATKKIDVYLPLHSSQDRLLPMTVVTMASARVQDLIGLICWQYTSEGREPKLNDNVSAYCLHIAEDDGEVDTDFPPLDSNEPIHKFGFSTLALVEKYSSPGLTSKESLFVRINAAHGFSLIQVDNTKVTMKE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAPKAP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Sin1/MAPKAP1 Protein (NBP1-89569PEP)
Publications
Read Publication using NBP1-89569.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Sin1/MAPKAP1 Antibody

  • JC310
  • MAPKAP1
  • MEKK2-interacting protein 1
  • MGC2745
  • MIP1
  • MIP1SIN1SIN1b
  • Mitogen-activated protein kinase 2-associated protein 1
  • mitogen-activated protein kinase associated protein 1
  • mSIN1
  • ras inhibitor MGC2745
  • SAPK-interacting protein 1
  • Sin1
  • SIN1g
  • stress-activated map kinase interacting protein 1
  • Stress-activated map kinase-interacting protein 1
  • stress-activated protein kinase-interacting 1
  • target of rapamycin complex 2 subunit MAPKAP1
  • TORC2 subunit MAPKAP1

Background

Sin1 is a novel component of the eukaryotic SAPK pathway. Sin1 and the H3 and H4 histones interact genetically, with the C terminus of Sin1 physically associating with components of the SWI-SNF complex. This interaction is blocked by the N-terminal half of the protein in full length Sin1. It has been proposed that Sin1 acts as a regulatable bridge between the SWI-SNF complex and the nucleosome Sin1 is also required for effective transcription via the AP-1 factor Pap1, but does not prevent its nuclear translocation. Sin1 is a novel component of the eukaryotic SAPK pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB600-607
Species: Hu, Rt
Applications: ELISA, IHC, IHC-P, WB
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
NBP1-51645
Species: Hu, Mu, Pm
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP2-22356
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
NBP2-24837
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
AF4004
Species: Hu, Mu, Rt
Applications: IHC, WB
NBP1-89865
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-24503
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, WB
MAB6210
Species: Hu
Applications: Simple Western, WB
DRN00B
Species: Hu
Applications: ELISA
DCP00
Species: Hu
Applications: ELISA
M6000B
Species: Mu
Applications: ELISA
NBP1-76573
Species: Hu, Mu
Applications: ELISA, ICC, ICC/IF, IF, WB
NBP2-45861
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP2-92773
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP2-19804
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB

Publications for Sin1/MAPKAP1 Antibody (NBP1-89569)(1)

Reviews for Sin1/MAPKAP1 Antibody (NBP1-89569) (0)

There are no reviews for Sin1/MAPKAP1 Antibody (NBP1-89569). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Sin1/MAPKAP1 Antibody (NBP1-89569) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Sin1/MAPKAP1 Products

Research Areas for Sin1/MAPKAP1 Antibody (NBP1-89569)

Find related products by research area.

Blogs on Sin1/MAPKAP1

There are no specific blogs for Sin1/MAPKAP1, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Sin1/MAPKAP1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol MAPKAP1