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Siglec-8 Antibody

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Western Blot: Siglec-8 Antibody [NBP1-06078] - (0.3ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: Siglec-8 Antibody [NBP1-06078] - (0.3ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Siglec-8 Antibody Summary

Immunogen
Peptide with sequence C-SYKSQLNYKTKQ corresponding to internal region according to NP_055257.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SIGLEC8
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/ml
Application Notes
WB: Approx. 45 kDa band observed in MOLT4 lysates is reported in scientific literature (PMID: 12609831).
Publications
Read Publications using NBP1-06078.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Siglec-8 Antibody

  • CD329 antigen
  • CDw329
  • MGC59785
  • SAF2
  • SAF2SAF-2
  • sialic acid binding Ig-like lectin 8
  • Sialoadhesin family member 2
  • Siglec8
  • Siglec-8
  • SIGLEC8L
  • SIGLEC8Lsialic acid-binding Ig-like lectin 8

Background

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Siglec-8 Antibody (NBP1-06078)(2)

We have publications tested in 1 application: WB.


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Product General Protocols

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Bioinformatics

Gene Symbol SIGLEC8
Entrez
Uniprot