![Knockdown Validated: MAZ Antibody [NB100-86984] - Western blotting was performed to detect the protein expression of MAZ in GC cells treated with ctrl or shMAZ in an acidic microenvironment. Image collected and cropped by CiteAb from the following publication (//www.aging-us.com/lookup/doi/10.18632/aging.103013) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MAZ-Antibody-Knockdown-Validated-NB100-86984-img0007.jpg "Knockdown Validated: MAZ Antibody [NB100-86984] - Western blotting was performed to detect the protein expression of MAZ in GC cells treated with ctrl or shMAZ in an acidic microenvironment. Image collected and cropped by CiteAb from the following publication (//www.aging-us.com/lookup/doi/10.18632/aging.103013) licensed under a CC-BY license.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, IHC, IP, ChIP, KD |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 427 and 477 of human MYC-associated zinc finger protein using the numbering given in entry BAA33064.1 (GeneID 4150). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MAZ |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 25749382). |
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| Publications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
![Immunohistochemistry PTH Antibody (918462) [Unconjugated]](https://images.novusbio.com/images2/PTH_MAB7665_Immunohistochemistry_16650.jpg)
![Western Blot PTH Antibody (918462) [Unconjugated]](https://images.novusbio.com/images2/PTH_MAB7665_Western_Blot_16656.jpg)
| Publications using NB100-86984 | Applications | Species |
|---|---|---|
| Chen Y, Li H, Liu C et al. MAZ-LINC00645-GP73 Axis Promotes Hepatocellular Carcinoma Proliferation and Metastasis Research Square 2020-08-28 (Chemotaxis, Human) | Chemotaxis | Human |
| Xingjie Ren, Han Yang, Jovia L Nierenberg, Yifan Sun, Jiawen Chen, Cooper Beaman, Thu Pham, Mai Nobuhara, Maya Asami Takagi, Vivek Narayan, Yun Li, Elad Ziv, Yin Shen High-throughput PRIME-editing screens identify functional DNA variants in the human genome. Molecular cell 2023-12-25 [PMID: 38134886] | ||
| Wang Y, Sun L et al. Inhibiting Forkhead box K1 induces autophagy to reverse epithelial-mesenchymal transition and metastasis in gastric cancer by regulating Myc-associated zinc finger protein in an acidic microenvironment. Aging (Albany NY) 2020-08-04 [PMID: 32268297] (WB, Human) | WB | Human |
| Franz H, Greschik H, Willmann D et al. The histone code reader SPIN1 controls RET signaling in liposarcoma. Oncotarget 2015-03-10 [PMID: 25749382] (Chemotaxis) | Chemotaxis |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | MAZ |
| Entrez |
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| Uniprot |
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![Western Blot: MAZ Antibody [NB100-86984] - Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAZ antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/MAZ-Antibody-Western-Blot-NB100-86984-img0005.jpg "Western Blot: MAZ Antibody [NB100-86984] - Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAZ antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: MAZ Antibody [NB100-86984] - FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit antiMAZ/SAF-1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MAZ-Antibody-Immunohistochemistry-Paraffin-NB100-86984-img0003.jpg "Immunohistochemistry-Paraffin: MAZ Antibody [NB100-86984] - FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit antiMAZ/SAF-1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![Immunoprecipitation: MAZ Antibody [NB100-86984] - Detection of human MAZ by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MAZ antibody NB100-86984 (lot NB100-86984-2) used for IP at 6 ug per reaction. MAZ was also immunoprecipitated by a previous lot of this antibody (lot NB100-86984-1) and another rabbit anti-MAZ antibody. For blotting immunoprecipitated MAZ, NB100-86984 was used at 1 ug/ml. Detection: . Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/MAZ-Antibody-Immunoprecipitation-NB100-86984-img0006.jpg "Immunoprecipitation: MAZ Antibody [NB100-86984] - Detection of human MAZ by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MAZ antibody NB100-86984 (lot NB100-86984-2) used for IP at 6 ug per reaction. MAZ was also immunoprecipitated by a previous lot of this antibody (lot NB100-86984-1) and another rabbit anti-MAZ antibody. For blotting immunoprecipitated MAZ, NB100-86984 was used at 1 ug/ml. Detection: . Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: MAZ Antibody [NB100-86984] - MAZ is a new transcription activation target of FOXK1. (A) Analysis of the 9969 overlapping FOXK1 loci for overrepresented sequence elements identified significantly enriched FOXK1-like DNA-binding motifs, & the results showed that 12.92% of the FOXK1 genomic loci contained at least one of the motifs. (B) Potential binding sites for FOXK1 at the 5'-UTR of MAZ. (C) FOXK1 & MAZ coexpression in the TCGA GC dataset was analyzed by querying the open database ChIPBase v2.0 (r: 0.3449, p = 5.1e-14). The mRNA (D) & protein (E) levels of MAZ in MGC803 & AGS cells were attenuated by the silencing of FOXK1. The data are presented as the means ± S.D.s from three independent experiments. ** P < 0.01, *** P < 0.001, & **** P < 0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32268297), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-86984_rabbit-polyclonal-maz-antibody-310202415382431.jpg "Western Blot: MAZ Antibody [NB100-86984] - MAZ is a new transcription activation target of FOXK1. (A) Analysis of the 9969 overlapping FOXK1 loci for overrepresented sequence elements identified significantly enriched FOXK1-like DNA-binding motifs, & the results showed that 12.92% of the FOXK1 genomic loci contained at least one of the motifs. (B) Potential binding sites for FOXK1 at the 5'-UTR of MAZ. (C) FOXK1 & MAZ coexpression in the TCGA GC dataset was analyzed by querying the open database ChIPBase v2.0 (r: 0.3449, p = 5.1e-14). The mRNA (D) & protein (E) levels of MAZ in MGC803 & AGS cells were attenuated by the silencing of FOXK1. The data are presented as the means ± S.D.s from three independent experiments. ** P < 0.01, *** P < 0.001, & **** P < 0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32268297), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Chromatin Immunoprecipitation: MAZ Antibody [NB100-86984] - SPIN1 controls liposarcoma cell proliferation & survival by enhancing GDNF expression in cooperation with the transcription factor MAZ(A) Venn diagram depicting the overlap of SPIN1 & MAZ locations at gene promoters in T778 cells. (B) Intensity profiles for SPIN1 & MAZ occupancy of 5,680 gene promoters around the transcription start site (TSS –/+ 2000 bp). (C) Intensity profiles of presence of SPIN1, H3K4me3, & MAZ at the GDNF gene in T778 cells determined by ChIP-sequencing. (D, E) Immunoprecipitation (IP) of endogenous SPIN1 & MAZ from T778 cell extracts with antibodies against SPIN1 or MAZ as indicated. (F, G) Quantitative RT-PCR analysis of MAZ & GDNF expression in T778 cells stably transfected with control miRNA (miCtrl) or miRNA directed against MAZ [miMAZ(1)] (F) or MAZ expression plasmid (MAZ OE) (G) Expression of miMAZ or MAZ was induced by doxycycline. Uninduced cells served as control. (F, G) Error bars represent +/– SEM, *p < 0.05, **p < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25749382), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-86984_rabbit-polyclonal-maz-antibody-31020241628495.jpg "Chromatin Immunoprecipitation: MAZ Antibody [NB100-86984] - SPIN1 controls liposarcoma cell proliferation & survival by enhancing GDNF expression in cooperation with the transcription factor MAZ(A) Venn diagram depicting the overlap of SPIN1 & MAZ locations at gene promoters in T778 cells. (B) Intensity profiles for SPIN1 & MAZ occupancy of 5,680 gene promoters around the transcription start site (TSS –/+ 2000 bp). (C) Intensity profiles of presence of SPIN1, H3K4me3, & MAZ at the GDNF gene in T778 cells determined by ChIP-sequencing. (D, E) Immunoprecipitation (IP) of endogenous SPIN1 & MAZ from T778 cell extracts with antibodies against SPIN1 or MAZ as indicated. (F, G) Quantitative RT-PCR analysis of MAZ & GDNF expression in T778 cells stably transfected with control miRNA (miCtrl) or miRNA directed against MAZ [miMAZ(1)] (F) or MAZ expression plasmid (MAZ OE) (G) Expression of miMAZ or MAZ was induced by doxycycline. Uninduced cells served as control. (F, G) Error bars represent +/– SEM, *p < 0.05, **p < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25749382), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![COCO/DAND5 [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "COCO/DAND5 [Unconjugated]")
![N/A PSG1 [HRP]](https://images.novusbio.com/images2/DATA_PSG1_DPSG10_ELISA_797.jpg)
![N/A PSG1 [HRP]](https://images.novusbio.com/images2/PSG-1_DPSG10_ELISA_164.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_871.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_872.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/VEGF_DVE00_ELISA_208.jpg)
![Immunocytochemistry EGR1 Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af2818_human-egr1-affinity-purified-polyclonal-ab-immunocytochemistry-3102024831..jpg)
![Western Blot c-jun [p Ser63] Antibody (1018Y) [Unconjugated]](https://images.novusbio.com/images2/cJun_MAB8930_Western_Blot_18923.jpg)
![Immunocytochemistry c-jun [p Ser63] Antibody (1018Y) [Unconjugated]](https://images.novusbio.com/images2/cJun_MAB8930_Immunocytochemistry__Immunofluorescence_18945.jpg)
![Simple Western c-jun [p Ser63] Antibody (1018Y) [Unconjugated]](https://images.novusbio.com/images2/cJun_MAB8930_Simple_Western_19175.jpg)
![Immunocytochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg)
![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")