SHMT1 Antibody

Images

 
Immunohistochemistry-Paraffin: SHMT1 Antibody [NBP3-17022] - Analysis in human liver and cerebral cortex tissues using Anti-SHMT1 antibody. Corresponding SHMT1 RNA-seq data are presented for the same tissues.
Western Blot: SHMT1 Antibody [NBP3-17022] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil
Immunohistochemistry-Paraffin: SHMT1 Antibody [NBP3-17022] - Staining of human cerebral cortex shows low expression as expected.
Immunohistochemistry-Paraffin: SHMT1 Antibody [NBP3-17022] - Staining of human liver shows high expression.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

SHMT1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LQIQSDTGVRATLKEFKERLAGDKYQAAVQALREEVESFASLFPLPGLPDF
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SHMT1
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for SHMT1 Antibody

  • 14 kDa protein
  • CSHMT
  • cytoplasmic serine hydroxymethyltransferase
  • EC 2.1.2.1
  • Glycine hydroxymethyltransferase
  • MGC15229
  • MGC24556
  • serine hydroxymethyltransferase 1 (soluble)
  • serine hydroxymethyltransferase, cytosolic
  • Serine methylase
  • SHMT
  • SHMT1
  • SHMTEC 2.1.2.1

Background

SHMT1 encodes the cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative splicing of this gene results in 2 transcript variants encoding 2 different isoforms. Additional transcript variants have been described, but their biological validity has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SHMT1