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SHBG Antibody [Biotin]

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SHBG was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human SHBG Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2656) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
SHBG was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human SHBG Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2656) at 15 µg/mL overnight at 4 °C. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Unconjugated
AF2656
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF2656
Carrier Free
AF2656
Carrier Free
AF2656-SP
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SHBG Antibody [Biotin] Summary

Specificity
Detects human SHBG in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SHBG
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Readout System

Packaging, Storage & Formulations

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SHBG Antibody [Biotin]

  • ABP
  • ABPTestosterone-estrogen-binding globulin
  • androgen binding protein
  • MGC126834
  • MGC138391
  • SBP
  • sex hormone-binding globulin
  • Sex steroid-binding protein
  • SHBG
  • TEBG
  • Testis-specific androgen-binding protein
  • testosterone-binding beta-globulin
  • Testosterone-estradiol-binding globulin

Background

Human SHBG (also known as ABP or androgen-binding protein) is a variably glycosylated, secreted non-disulfide linked homodimer that belongs to the SHBG family. Members of this small family have tandem repeats of 170 amino acid (aa) long laminin alpha chain G-like domains. Each SHBG monomer is a 47-53 kDa, 373 aa glycoprotein that contains one steroid-binding site in its N-terminal G-like domain. Male and female sex hormones are bound with equal affinity. SHBG is synthesized by the liver and circulates in blood; ABP (SHBG in the testis) is synthesized by Sertoli cells and circulates in the male reproductive system. Human SHBG shares approximately 67% aa sequence identity with mouse and rat SHBG.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SHBG
Uniprot