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sFRP-4 Antibody

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Western blot shows lysates of OVCAR-3 human ovarian carcinoma cell line, human ovarian cancer tissue, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human sFRP-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1827) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for sFRP-4 at approximately 53-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Western blot shows lysates of OVCAR-3 human ovarian carcinoma cell line, human ovarian cancer tissue, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human sFRP-4 Antigen ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF1827-SP
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sFRP-4 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human sFRP-4
Ala22-Val346
Accession # AAC04617
Specificity
Detects human sFRP-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse (rm) sFRP-4 is observed, and less than 5% cross-reactivity with recombinant human (rh) sFRP-3, rhsFRP-1, and rmsFRP-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SFRP4
Purity Statement
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
Publications
Read Publications using AF1827.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for sFRP-4 Antibody

  • DDC-4
  • Frizzled protein, human endometrium
  • FRP-4
  • FRP-AP
  • frpHE
  • FRPHEMGC26498
  • FrzB2
  • secreted frizzled-related protein 4
  • secreted frizzled-related protein 4; secreted frizzled-related protein 4
  • sFRP4
  • sFRP-4

Background

sFRP-4, also known as DDC-4, Frp, FRPHE and Secreted Frizzled Related Protein 4, is a 50-55 kDa glycoprotein expressed in brain, kidney, lung, ovary, prostate, mammary gland, and endometrium (1, 2). This protein shows complex functions with respect to cell survival: it is up‑regulated with apoptosis during ovulation (3), regulates apoptosis in chondrocytes (4), and promotes apoptosis in mammary glands when expressed in transgenic mice (5). On the other hand, sFRP-4 can also act to enhance growth as it is up‑regulated in endometrial and breast carcinomas (6, 7). Since it is not detected in other carcinomas such as the ovary, colon, and pancreas, this suggest that its role in cancer is likely to be tissue dependent (6). In addition sFRP-4 is characterized as a circulating phosphaturic factor expressed by tumors associated with osteomalacia that antagonizes renal Wnt signaling (7). Of all the secreted frizzled related proteins, sFRP-4 is most closely related to sFRP-3 (1). Mouse and human sFRP-4 proteins share 92% aa identity.

  1. Jones, S.E. and C. Jomary (2002) Bioessays 24:811.
  2. Wolf, V. et al. (1997) FEBS Letters 417:385.
  3. Drake, J.M. et al. (2003) Apoptosis 8:389.
  4. James, I.E. et al. (2000) Osteoarthritis & Cartilage 8:452.
  5. Lacher, M.D. et al. (2003) Cell Death Differ. 10:528.
  6. Abu-Jawdeh, G. et al. (1999) Lab Investigation 79:439.
  7. Wong, S.C.C. et al. (2002) J. Pathology 196:145.
  8. Berndt, T. et al. (2003) J. Clin. Invest. 112:785.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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sFRP-4 Antibody
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Publications for sFRP-4 Antibody (AF1827)(2)


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Bioinformatics

Gene Symbol SFRP4
Uniprot