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SF3A2 Antibody

Images

 
Independent Antibodies: Western Blot: SF3A2 Antibody [NBP2-47281] - Analysis using Anti-SF3A2 antibody NBP2-47281 (A) shows similar pattern to independent antibody NBP2-48775 (B).
Immunocytochemistry/ Immunofluorescence: SF3A2 Antibody [NBP2-47281] - Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: SF3A2 Antibody [NBP2-47281] - Staining of human kidney.
Genetic Strategies: Western Blot: SF3A2 Antibody [NBP2-47281] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.
Immunohistochemistry: SF3A2 Antibody [NBP2-47281] - Staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.
Independent Antibodies: Immunohistochemistry-Paraffin: SF3A2 Antibody [NBP2-47281] - Staining of human cerebral cortex, colon, kidney and testis using Anti-SF3A2 antibody NBP2-47281 (A) shows similar protein ...read more
Immunohistochemistry-Paraffin: SF3A2 Antibody [NBP2-47281] - Staining of human testis.
Immunohistochemistry-Paraffin: SF3A2 Antibody [NBP2-47281] - Staining of human cerebral cortex.
Immunohistochemistry-Paraffin: SF3A2 Antibody [NBP2-47281] - Staining of human colon.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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SF3A2 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: SSSESNRDRRERLRQLALETIDINKDPYFMKNHLGSYECKLCLTLHNNEGSYLAHTQGKKHQTNLARRAAKEAKEAPAQPAPEKVKVE
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SF3A2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
SF3A2 Protein (NBP2-47281PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SF3A2 Antibody

  • pre-mRNA splicing factor SF3A, subunit 2
  • PRP11
  • PRPF11
  • SAP 62
  • SAP62Prp11
  • SF3a66splicing factor 3a, subunit 2, 66kD
  • spliceosome associated protein 62
  • Spliceosome-associated protein 62
  • splicing factor 3A subunit 2
  • splicing factor 3a, subunit 2, 66kDa

Background

SF3A2 encodes subunit 2 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 2 interacts with subunit 1 through its amino-terminus while the single zinc finger domain of subunit 2 plays a role in its binding to the 15S U2 snRNP. Subunit 2 may also function independently of its RNA splicing function as a microtubule-binding protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol SF3A2