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Serpin A8/Angiotensinogen Antibody [Unconjugated]

 (12 Publications)
 
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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Serpin A8/Angiotensinogen Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3156) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Serpin A8/Angiotensinogen at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Serpin A8/Angiotensinogen Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Serpin A8/Angiotensinogen was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 1.7 µg/mL Goat Anti-Human Serpin A8/Angiotensinogen Antigen Affinity-purified Polyclonal Antibody ...read more
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Serpin A8/Angiotensinogen at approximately 64 kDa (as indicated) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF3156
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Catalog# & Formulation Size Price
Carrier Free
AF3156-SP
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Serpin A8/Angiotensinogen Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Serpin A8/Angiotensinogen
Asp34-Ala485
Accession # ABM85122
Specificity
Detects human Serpin A8/Angiotensinogen in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Serpin A8/Angiotensinogen is observed, and less than 5% cross-reactivity with recombinant human Serpin A1, A4, A5, F2, G1, I1, and I2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
AGT
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 2-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF3156 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serpin A8/Angiotensinogen Antibody [Unconjugated]

  • AGT
  • alpha-1 antiproteinase, antitrypsin
  • Angiotensin II
  • angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
  • Angiotensinogen
  • ANHU
  • Aogen
  • FLJ92595
  • FLJ97926
  • PAT
  • pre-angiotensinogen
  • serine (or cysteine) proteinase inhibitor
  • Serpin A8
  • SERPINA8angiotensin I

Background

Serpin A8 (serine proteinase inhibitor-clade A #8; also angiotensinogen/ANGT) is a secreted, 52-62 kDa glycoprotein member of the clade F-subfamily, serpin superfamily of protease inhibitors.  It is expressed by neurons and hepatocytes, and undergoes extracellular cleavage by renin to create a ten amino acid (aa) peptide termed Ang/angiotensin I.  This inactive peptide is further cleaved by ACE on the endothelial cell membrane to create bioactive Ang II.  Ang II induces vascoconstriction and aldosterone release.  Mature human angiotensinogen is 452 aa in length (aa 34-485).  It contains Ang I (aa 34-43) that is cleaved to create Ang II (aa 34-41).  Angiotensinogen may circulate in a 200 kDa complex with major basic protein (MBP), or as part of a larger 300 kDa complex with MBP and complement C3dg.  Over aa 34-485, human serpin A8 shares 61% aa identity with mouse serpin A8.        

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Serpin A8/Angiotensinogen Antibody (AF3156)(12)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ELISA Capture, IHC-Fr, IHC-P, Western Blot.


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ELISA Capture
(1)
IHC-Fr
(1)
IHC-P
(1)
Western Blot
(1)
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Human
(2)
Mouse
(2)
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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using AF3156 Applications Species
Mravinacová, S;Alanko, V;Bergström, S;Bridel, C;Pijnenburg, Y;Hagman, G;Kivipelto, M;Teunissen, C;Nilsson, P;Matton, A;Månberg, A; CSF protein ratios with enhanced potential to reflect Alzheimer's disease pathology and neurodegeneration Molecular neurodegeneration 2024-02-13 [PMID: 38350954] (ELISA Capture, Human) ELISA Capture Human
Liu HH, Li XQ, Liu JF et al. miR-6869-5p Transported by Plasma Extracellular Vesicles Mediates Renal Tubule Injury and Renin-Angiotensin System Activation in Obesity Frontiers in Medicine 9/8/2021 [PMID: 34568382]
Liu HH, Li XQ, Liu JF et al. miR-6869-5p Transported by Plasma Extracellular Vesicles Mediates Renal Tubule Injury and Renin-Angiotensin System Activation in Obesity Frontiers in Medicine 2021-09-08 [PMID: 34568382]
Sarah J Delforce Expression of renin angiotensin system (RAS) components in endometrial cancer Endocr Connect, 2016-12-12;0(0):. 2016-12-12 [PMID: 27956412] (IHC-P, Human) IHC-P Human
Andrew J R White, Sarat C Cheruvu, Maria Sarris, Surabhi S Liyanage, Eugenie Lumbers, Jeanie Chui et al. Expression of classical components of the renin-angiotensin system in the human eye Journal of the Renin-Angiotensin-Aldosterone System 3/1/2015 [PMID: 25287897]
A Casselbrant, S Kostic, H Lönroth The muscular expression of RAS in patients with achalasia Journal of the Renin-Angiotensin-Aldosterone System 9/1/2015 [PMID: 24468662]
Tang C, Wei W, Hanes M, Liu L Hepatocarcinogenesis driven by GSNOR deficiency is prevented by iNOS inhibition. Cancer Res, 2013-02-25;73(9):2897-904. 2013-02-25 [PMID: 23440427] (Western Blot, Mouse) Western Blot Mouse
Pablo Garrido-Gil, Rita Valenzuela, Begoña Villar-Cheda, Jose L. Lanciego, Jose L. Labandeira-Garcia Expression of angiotensinogen and receptors for angiotensin and prorenin in the monkey and human substantia nigra: an intracellular renin–angiotensin system in the nigra Brain Structure and Function 3/1/2013 [PMID: 22407459]
K.G. Pringle, M.A. Tadros, R.J. Callister, E.R. Lumbers The expression and localization of the human placental prorenin/renin-angiotensin system throughout pregnancy: Roles in trophoblast invasion and angiogenesis? Placenta 12/1/2011 [PMID: 22018415]
F.Z. Marques, K.G. Pringle, A. Conquest, J.J. Hirst, M.A. Markus, M. Sarris et al. Molecular characterization of renin-angiotensin system components in human intrauterine tissues and fetal membranes from vaginal delivery and cesarean section Placenta 3/1/2011 [PMID: 21215447]
Show All 12 Publications.

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Bioinformatics

Gene Symbol AGT
Uniprot