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Serpin A6/Cortisol Binding Globulin Antibody [Unconjugated]

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Western blot shows lysates of human plasma and human serum. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Serpin A6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9026) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Serpin A6 at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human plasma and human serum. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Serpin A6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9026) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF9026-SP
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Serpin A6/Cortisol Binding Globulin Antibody [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Serpin A6
Met23-Val405 (Ser246Ala)
Accession # P08185
Specificity
Detects human Serpin A6 in direct ELISAs and Western Blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Serpin A1, rhSerpin A3, and rhSerpin A4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
SERPINA6
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serpin A6/Cortisol Binding Globulin Antibody [Unconjugated]

  • antitrypsin), member 6
  • CBG
  • CBGcorticosteroid-binding globulin
  • corticosteroid binding globulin
  • member 6
  • serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase
  • Serpin A6
  • serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)
  • transcortin

Background

Serpin A6, also known as Corticosteroid-binding Globulin/CBG and Transcortin, is a 45‑60 kDa secreted glycoprotein member of the clade A serpin family of protease inhibitors. Although it is a serpin family member, it shows no protease inhibition. Serpin A6 is produced principally by hepatocytes, but is also expressed by hypothalamic neurons and anterior pituitary endocrine cells. Serpin A6 transports progesterone and > 90% of circulating cortisol, and likely has three functions: first it can be cleaved, releasing cortisol to the tissues; second, it regulates levels of free hormone available over time; and third, it likely binds to specific cell-surface receptors when complexed to cortisol. Human Serpin A6 is 405 amino acids (aa) in length. Over aa 23‑396, human Serpin A6 shares 57% and 60% aa sequence identity with mouse and rat Serpin A6, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SERPINA6
Uniprot