![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Analysis using HRP conjugate of NB600-602. Lane 1: reduced Alpha-1anti-Trypsin. Lane 2: none. Load: 1ug per lane. Primary antibody: Alpha-1-Anti-Trypsin antibody at 1:3000 for overnight at 4C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 1.5 hr at RT. Block: incubated with blocking buffer for Alpha-1-AntiTrypsin. Other band(s): none.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Western-Blot-NB600-602-img0014.jpg "Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Analysis using HRP conjugate of NB600-602. Lane 1: reduced Alpha-1anti-Trypsin. Lane 2: none. Load: 1ug per lane. Primary antibody: Alpha-1-Anti-Trypsin antibody at 1:3000 for overnight at 4C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 1.5 hr at RT. Block: incubated with blocking buffer for Alpha-1-AntiTrypsin. Other band(s): none.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, IP |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | LYOPH |
| Description | This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified a1-Anti-Trypsin [Human Plasma] Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | Serpin A1/alpha 1-Antitrypsin [Human Plasma] (Uniprot: P01009) |
| Localization | Secreted |
| Specificity | Cross reactivity against Serpin A1/alpha 1-Antitrypsin from other sources is unknown. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | SERPINA1 |
| Purity | Multi-step |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This product has been tested by ELISA and western blot. This product has been assayed against 1.0 ug of Alpha-1-Anti-trypsin [Human Plasma] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested. |
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| Storage | Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Concentration | LYOPH |
| Purity | Multi-step |
| Reconstitution Instructions | Reconstitute with 100 ul deionized water (or equivalent) |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Serpin A1/alpha 1-Antitrypsin Antibody (NB600-602)Find related products by research area.
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![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Lane 1: Albumin depleted human serum. Lane 2: none. Load: 10 ug per lane. Primary antibody: none. Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 52 kDa, 52 kDa for Alpha-1-Anti-Trypsin. Other band(s): none](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Western-Blot-NB600-602-img0010.jpg "Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Lane 1: Albumin depleted human serum. Lane 2: none. Load: 10 ug per lane. Primary antibody: none. Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 52 kDa, 52 kDa for Alpha-1-Anti-Trypsin. Other band(s): none")
![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Lane 1: Alpha-1anti-Trypsin under reducing conditions. Lane 2: none. Load: 1 ug per lane. Primary antibody: Alpha-1anti-Trypsin antibody at 1:3000 for overnight at 4C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 45 min at RT. Block: incubated with blocking buffer for alpha-1 Anti-Trypsin. Other band(s): none](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Western-Blot-NB600-602-img0011.jpg "Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Lane 1: Alpha-1anti-Trypsin under reducing conditions. Lane 2: none. Load: 1 ug per lane. Primary antibody: Alpha-1anti-Trypsin antibody at 1:3000 for overnight at 4C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 45 min at RT. Block: incubated with blocking buffer for alpha-1 Anti-Trypsin. Other band(s): none")
![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Lane 1: molecular weight. Lane 2: Alpha-1-Anti-Trypsin (red), Rabbit anti-Transferrin, and Mouse-a-GST were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 35 ug per lane. Primary antibody: Each primary antibody at 1:1000 for overnight at 4C. Secondary antibody: DyLight549 Donkey anti-Rabbit IgG (green), DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000 for 30 min at RT. Block: at 30 min RT](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Western-Blot-NB600-602-img0012.jpg "Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Lane 1: molecular weight. Lane 2: Alpha-1-Anti-Trypsin (red), Rabbit anti-Transferrin, and Mouse-a-GST were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 35 ug per lane. Primary antibody: Each primary antibody at 1:1000 for overnight at 4C. Secondary antibody: DyLight549 Donkey anti-Rabbit IgG (green), DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000 for 30 min at RT. Block: at 30 min RT")
![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Lane 1: molecular weight. Lane 2: Human transferrin (green); Alpha 1 anti trypsin (red); and Human IgG (Blue) in a multiplex fluorescent western blot of human serum. Load: 35 ug per lane. Primary antibody: Each primary antibody was diluted to 1:1000 for overnight at 4C. Secondary antibody: Rabbit IgG (H&L) Antibody DyLight 649 Conjugated (green), Goat IgG (H&L) Antibody DyLight 549 Conjugated (red), Mouse IgG (H&L) Antibody DyLight 488 Conjugated (blue)secondary antibody at 1:10,000 for 1hr at 4 degrees. Block: IRdye blocking buffer overnight at 4C](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Western-Blot-NB600-602-img0013.jpg "Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Lane 1: molecular weight. Lane 2: Human transferrin (green); Alpha 1 anti trypsin (red); and Human IgG (Blue) in a multiplex fluorescent western blot of human serum. Load: 35 ug per lane. Primary antibody: Each primary antibody was diluted to 1:1000 for overnight at 4C. Secondary antibody: Rabbit IgG (H&L) Antibody DyLight 649 Conjugated (green), Goat IgG (H&L) Antibody DyLight 549 Conjugated (red), Mouse IgG (H&L) Antibody DyLight 488 Conjugated (blue)secondary antibody at 1:10,000 for 1hr at 4 degrees. Block: IRdye blocking buffer overnight at 4C")
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