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Semaphorin 3B Antibody (904201) [Unconjugated]

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Western blot shows lysates of NS0 mouse myeloma cell line transfected with mouse Semaphorin 3B. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Semaphorin 3B Monoclonal Antibody (Catalog # MAB5440) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Semaphorin 3B at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of NS0 mouse myeloma cell line transfected with mouse Semaphorin 3B. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Semaphorin 3B Monoclonal Antibody (Catalog # MAB5440) followed ...read more
Semaphorin 3B was detected in immersion fixed NS0 mouse myeloma cell line transfected with mouse Semaphorin 3B (upper panel) or irrelevant transfectant (lower panel) using Rat Anti-Mouse Semaphorin 3B Monoclonal ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ICC/IF
Clone
904201
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB5440-SP
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Semaphorin 3B Antibody (904201) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 3B 
Pro26-Pro722
Accession # Q62177
Specificity
Detects mouse Semaphorin 3B in direct ELISAs.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
SEMA3B
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 0.5 ug/mL
Publications
Read Publication using
MAB5440 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Semaphorin 3B Antibody (904201) [Unconjugated]

  • (semaphorin) 3B
  • LUCA-1
  • Sema A(V)
  • sema domain, immunoglobulin domain (Ig), short basic domain, secreted
  • Sema V
  • SemA
  • SEMA3B
  • SEMA5
  • SEMA5FLJ34863
  • SEMAA
  • Semaphorin 3B
  • semaphorin A
  • semaphorin V
  • semaphorin-3B
  • semaphorin-V
  • SEMAV

Background

Sema3b (Semaphorin 3B) is a 748 aa secreted glycoprotein.  Mouse Sema3b shares 88% and 96% aa identity with human and rat Sema3b, respectively.  The semaphorin family has been demonstrated to play a role in axon guidance.  Additionally, Sema3b has been implicated in bone mass regulation and tumor suppression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Semaphorin 3B Antibody (MAB5440)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Western Blot.


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Bioinformatics

Gene Symbol SEMA3B
Uniprot