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SCAP2 Antibody

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Western Blot: SCAP2 Antibody [NB110-40659] - Analysis of SCAP2 in Jurkat lysate (35ug protein in RIPA buffer) using this antibody at 0.5ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: SCAP2 Antibody [NB110-40659] - Analysis of SCAP2 in Jurkat lysate (35ug protein in RIPA buffer) using this antibody at 0.5ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: SCAP2 Antibody [NB110-40659] - HEK293 overexpressing SKAP2 and probed with (mock transfection in first lane).

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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SCAP2 Antibody Summary

Immunogen
Peptide with sequence C-GLVPKAYIMEMYDI corresponding to C-Terminus according to NP_003921.2.
Predicted Species
Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SKAP2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.5 - 2 ug/ml
Application Notes
WB: Approx. 38 kDa band observed in Jurkat lysates (calculated MW of 41.2 kDa band according to NP_003921.2). In transfected HEK293 transiently expressing SKAP2 a band of approx. 45 kDa is observed. This band is not observed in the non-transfected HEK293. The calculated An additional band of unknown identity was also consistently observed in Jurkat at 28 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Control
SCAP2 Overexpression Lysate
Publications
Read Publication using NB110-40659.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SCAP2 Antibody

  • Fyn-associated phosphoprotein SKAP55 homologue
  • PRAP
  • RA70Pyk2/RAFTK-associated protein
  • Retinoic acid-induced protein 70
  • SAPSSKAP55 homolog
  • SCAP2MGC33304
  • SKAP55RSKAP-55HOM
  • SKAP-HOMMGC10411
  • src family associated phosphoprotein 2
  • Src family-associated phosphoprotein 2
  • src kinase associated phosphoprotein 2
  • src kinase-associated phosphoprotein 2
  • Src kinase-associated phosphoprotein 55-related protein
  • src kinase-associated phosphoprotein of 55-related protein
  • Src-associated adapter protein with PH and SH3 domains
  • src-associated adaptor protein

Background

The protein encoded by this gene belongs to the src family kinases. This protein is similar to the src kinase associated phosphoprotein 1. It is an adaptor protein that is thought to play an essential role in the src signaling pathway in various cells. It inhibits PTK2B/RAFTK activity and regulates alpha-synuclein phosphorylation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SCAP2 Antibody (NB110-40659)(1)


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Product General Protocols

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Video Protocols

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Control Lysate(s)

SCAP2 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SKAP2
Entrez
Uniprot