SATB2 Antibody (CL0276) - Azide and BSA Free

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Orthogonal Strategies: Analysis in human rectum and skeletal muscle tissues using NBP3-44079 antibody. Corresponding SATB2 RNA-seq data are presented for the same tissues.
Staining of human cerebral cortex shows strong nuclear positivity in neurons.
Staining of human colorectal cancer shows moderate to strong nuclear positivity in tumor cells.
Staining of human skeletal muscle shows no positivity in striated muscle fibers, as expected.
Staining of human rectum shows strong nuclear positivity in glandular cells.
Analysis in human cell line HEL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
CL0276
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH
Validated by:
       

Orthogonal Strategies

 

Order Details

SATB2 Antibody (CL0276) - Azide and BSA Free Summary

Immunogen
This antibody was generated using a recombinant protein sequence of Q9UPW6, with the exact immunogen sequence remaining proprietary.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SATB2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Lyophilized in 5% Trehalose.
Preservative
0.02% Sodium Azide
Concentration
LYOPH
Purity
Protein A purified
Reconstitution Instructions
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for SATB2 Antibody (CL0276) - Azide and BSA Free

  • DNA-binding protein SATB2
  • FLJ32076
  • KIAA1034FLJ21474
  • MGC119474
  • MGC119477
  • SATB family member 2
  • SATB homeobox 2
  • Special AT-rich sequence-binding protein 2

Background

SATB2 binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional SATB2 Products

Array NBP3-44079

Research Areas for SATB2 Antibody (NBP3-44079)

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Bioinformatics

Gene Symbol SATB2