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SAT1 Antibody - Azide and BSA Free

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Western Blot: SAT1 Antibody [NBP2-93325] - Analysis of extracts of THP-1 cells, using SAT1 at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free

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Catalog# & Formulation Size Price
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SAT1 Antibody - Azide and BSA Free Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-171 of human SAT1 (NP_002961.1). MAKFVIRPATAADCSDILRLIKELAKYEYMEEQVILTEKDLLEDGFGEHPFYHCLVAEVPKEHWTPEGHSIVGFAMYYFTYDPWIGKLLYLEDFFVMSDYRGFGIGSEILKNLSQVAMRCRCSSMHFLVAEWNEPSINFYKRRGASDLSSEEGWRLFKIDKEYLLKMATEE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SAT1
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Western Blot 1:500 - 1:1000
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for SAT1 Antibody - Azide and BSA Free

  • DC21
  • diamine acetyltransferase 1
  • diamine N-acetyltransferase 1
  • EC 2.3.1.57
  • KFSD
  • Polyamine N-acetyltransferase 1
  • Putrescine acetyltransferase
  • SAT
  • Spermidine/spermine N(1)-acetyltransferase 1
  • spermidine/spermine N1-acetyltransferase 1
  • spermidine/spermine N1-acetyltransferase alpha
  • spermidine/spermine N1-acetyltransferase
  • SSAT-1
  • SSATKFSDX

Background

SAT1--also known as Spermidine/spermine-N1-acetyltransferase (SSAT1)--is a short-lived polyamine catabolic enzyme which catalyzes the acetylation of spermidine and spermine. SAT1 is a highly regulated enzyme which allows a fine attenuation of the intracellular concentration of polyamines. Induction of SAT1 plays an important role in polyamine homoeostasis, since the N1-acetylated polyamines can be excreted or oxidized by acetylpolyamine oxidase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SAT1 Antibody (NBP2-93325) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for SAT1 Antibody (NBP2-93325). (Showing 1 - 1 of 1 FAQ).

  1. I need to radiolabel (iodinate) the SAT1 antibody. Which one should I use?
    • I would recommend NB110-41622 for you as it is a well validated antibody with very clean QC validation data, multiple applications/broad species reactivity. Additionally, this antibody has been cited by Liao et al. in The Journal of Biological Chemistry 2009;284(12):8174-8184 (https://www.jbc.org/article/S0021-9258(20)32529-1/fulltext).

Secondary Antibodies

 

Isotype Controls

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Research Areas for SAT1 Antibody (NBP2-93325)

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Bioinformatics

Gene Symbol SAT1