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RPA70 Antibody (4C4) - BSA Free

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Western Blot: RPA70 Antibody (4C4) [NBP2-37500] - Western blot analysis using RPA1 mouse mAb against HeLa (1), MCF-7 (2), K562(3), A431(4), and COS-7 (6) cell lysate.
Immunocytochemistry/ Immunofluorescence: RPA70 Antibody (4C4) [NBP2-37500] - Immunofluorescence analysis of HeLa cells using RPA1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry: RPA70 Antibody (4C4) [NBP2-37500] - Immunohistochemical analysis of paraffin-embedded prostate tissues using RPA1 mouse mAb with DAB staining.
Flow Cytometry: RPA70 Antibody (4C4) [NBP2-37500] - Flow cytometric analysis of Jurkat cells using RPA1 mouse mAb (green) and negative control (red).
Western Blot: RPA70 Antibody (4C4) [NBP2-37500] - Western blot analysis using RPA1 mAb against human RPA1 (AA: 308-513) recombinant protein. (Expected MW is 48.3 kDa)
Immunohistochemistry: RPA70 Antibody (4C4) [NBP2-37500] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using RPA1 mouse mAb with DAB staining.
ELISA: RPA70 Antibody (4C4) [NBP2-37500] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity Hu, Pm, PmSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
Clone
4C4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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Catalog# & Formulation Size Price
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RPA70 Antibody (4C4) - BSA Free Summary

Immunogen
Purified recombinant fragment of human RPA70 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RPA1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
68.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for RPA70 Antibody (4C4) - BSA Free

  • HSSB
  • MST075
  • MSTP075
  • REPA1RF-A protein 1
  • Replication factor A protein 1
  • replication protein A 70 kDa DNA-binding subunit
  • replication protein A1 (70kD)
  • replication protein A1, 70kDa
  • RF-A
  • RP-A
  • RPA70RP-A p70
  • Single-stranded DNA-binding protein

Background

RPA (Replication protein A) is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively). RPA is a single strand binding protein that is involved in replication, homologous recombination and nucleotide excision and repair. It colocalises with RAD51 on synapsed axes in meiosis, facilitating RAD51 function. Replication protein A acts as an auxiliary protein for DNA polymerases alpha and delta.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RPA1