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RPA14 Antibody (11.1)

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Western Blot: RPA14 Antibody (11.1) [NB100-159] - A: 30 ug A549 whole cell lysate/extract. B: 30 ug MCF-7 whole cell lysate/extract. Separated by 15% SDS-PAGE. RPA14 antibody [11.1] at 1:1000.
Immunohistochemistry-Paraffin: RPA14 Antibody (11.1) [NB100-159] - Human osteosarcoma section, 4% formaldehyde fixed. Incubated with the primary antibody for 10-15 hours. IHC-P image submitted by a verified customer ...read more
Western Blot: RPA14 Antibody (11.1) [NB100-159] - 30 ug of MCF7 whole cell lysate separated by 15% SDS-PAGE. Antibody at 1:500.
Immunohistochemistry-Paraffin: RPA14 Antibody (11.1) [NB100-159] - Mahlarvu xenograft. RPA14 antibody [11.1] dilution: 1:200. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IP
Clone
11.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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RPA14 Antibody (11.1) Summary

Immunogen
Full-length human RPA-14 expressed in E. coli..
Localization
Nuclear
Specificity
NB100-159 is specific for human and mouse RPA14.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RPA3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 0.1-2 ug/ml
  • Western Blot 0.1-2 ug/ml
Theoretical MW
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB100-159 in the following applications:

Publications
Read Publications using
NB100-159 in the following applications:

  • WB
    2 publications

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for RPA14 Antibody (11.1)

  • REPA3Replication factor A protein 3
  • replication protein A 14 kDa subunit
  • replication protein A3 (14kD)
  • replication protein A3, 14kDa
  • RF-A protein 3
  • RP-A p14
  • RPA14

Background

Replication protein A (RPA) is a single-stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 70, 32 and 14 kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination and nucleotide excision repair. RPA has been shown to be a target for DNA-dependent protein kinase (DNA-PK) ataxia telangiectasia-mutated gene (ATM) and a cyclin dependent protein kinase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RPA14 Antibody (NB100-159)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(2)
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Filter By Species
Human
(2)
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Showing Publications 1 - 8 of 8.
Publications using NB100-159 Applications Species
Calle A, Toribio-Serrano V, YANez-MO M, RamIrez M Embryonic trophectoderm secretomics reveals chemotactic migration and intercellular communication of endometrial and circulating MSCs in embryonic implantation Int J Mol Sci 2021-06-02 [PMID: 34073234]
Yoshihara T, Ishida M, Kinomura A et al. XRCC3 deficiency results in a defect in recombination and increased endoreduplication in human cells. EMBO J 2004-02-01 [PMID: 14749735] (WB, Human) WB Human
Meetei AR, Sechi S, Wallisch M et al. A multiprotein nuclear complex connects Fanconi anemia and Bloom syndrome. Mol Cell Biol 2003-05-01 [PMID: 12724401] (WB, Human) WB Human
Iftode, C, Daniely, Y, Borowiec, JA. Replication protein A (RPA): the eukaryotic SSB. Crit Rev Biochem Mol Biol 34:141-180. 1999-01-01 [PMID: 10473346]
Bochkarev, A, Bochkareva, E, Frappier, L, Edwards, AM. The crystal structure of the complex of replication protein A subunits RPA32 RPA14 reveals a mechanism for single-stred DNA binding. EMBO J 18:4498-4504. 1999-01-01 [PMID: 10449415]
Dong, J, Park, JS, Lee, SH. In vitro analysis of the zinc-finger motif in human replication protein A. Biochem J 337:311-317. 1999-01-01 [PMID: 9882630]
Sibenaller, ZA, Sorensen, BR, Wold, MS. The 32- 14-kilodalton subunits of replication protein A are responsible for species-specific interactions with single-stred DNA. Biochemistry 37:12496-12506. 1998-01-01 [PMID: 9730822]
Stigger, E, Drissi, R, Lee, SH. Functional analysis of human replication protein A in nucleotide excision repair. J Biol Chem 273:9337-9343. 1998-01-01 [PMID: 9535929]

Review for RPA14 Antibody (NB100-159) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-159:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
IHC-P Human 10/10/2021
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedOsteosarcoma
SpeciesHuman
Lot11.1

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Verified Customer
10/10/2021
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol RPA3
Entrez
Uniprot