![Immunocytochemistry/Immunofluorescence: ROR gamma/RORC/NR1F3 Antibody (RORC/2941) [NBP3-08314] - Immunofluorescence staining of PFA-fixed MOLT4 cells with ROR gamma/RORC/NR1F3 Mouse Monoclonal Antibody (RORC/2941) followed by goat anti- Mouse IgG-CF488. Nuclei counterstained with RedDot.](http://images.novusbio.com/fullsize/ROR-gamma-RORC-NR1F3-Antibody-RORC-2941-Immunocytochemistry-Immunofluorescence-NBP3-08314-img0002.jpg "Immunocytochemistry/Immunofluorescence: ROR gamma/RORC/NR1F3 Antibody (RORC/2941) [NBP3-08314] - Immunofluorescence staining of PFA-fixed MOLT4 cells with ROR gamma/RORC/NR1F3 Mouse Monoclonal Antibody (RORC/2941) followed by goat anti- Mouse IgG-CF488. Nuclei counterstained with RedDot.")
| Reactivity | HuSpecies Glossary |
| Applications | Flow, ICC/IF, IHC, MA |
| Clone | RORC/2941 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07564). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Immunogen | Recombinant full-length human ROR gamma/RORC/NR1F3 protein (Uniprot: P51449) |
| Epitope | aa1-50 |
| Localization | Nuclear |
| Specificity | This monoclonal antibody recognizes a protein of 63kDa, identified as ROR-C. Its epitope maps in between aa1-50. The nuclear orphan receptors ROR are members of the nuclear hormone receptor superfamily. Members of this family act by directly associating with DNA sequences known as hormone response elements (HREs) and typically bind DNA as either homo- or heterodimers. RORalpha and RORgamma are unique in that they bind DNA as monomers. RORalpha has multiple isoforms that share common DNA and putative ligand-binding domains, but differ in their amino terminal domains, which are generated by alternative RNA processing. RORgamma comprises a 560 amino acid protein that shares 50% amino acid identity with RORalpha and is most highly expressed in skeletal muscle. Although these proteins are considered orphan receptors, due to a lack of defined ligands, experimental evidence has shown that melatonin may be the natural ligand for these nuclear receptors. |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RORC |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Flow Cytometry: 1-2ug/million cells 3% PFA-fixed cells |
| Theoretical MW | 63 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for ROR gamma/RORC/NR1F3 Antibody (NBP3-08314)Find related products by research area.
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| Gene Symbol | RORC |
![Immunohistochemistry-Paraffin: ROR gamma/RORC/NR1F3 Antibody (RORC/2941) [NBP3-08314] - Formalin-fixed, paraffin-embedded human kidney stained with ROR gamma/RORC/NR1F3 Mouse Monoclonal Antibody (RORC/2941).](http://images.novusbio.com/fullsize/ROR-gamma-RORC-NR1F3-Antibody-RORC-2941-Immunohistochemistry-Paraffin-NBP3-08314-img0004.jpg "Immunohistochemistry-Paraffin: ROR gamma/RORC/NR1F3 Antibody (RORC/2941) [NBP3-08314] - Formalin-fixed, paraffin-embedded human kidney stained with ROR gamma/RORC/NR1F3 Mouse Monoclonal Antibody (RORC/2941).")
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![Immunohistochemistry-Paraffin: ROR gamma/RORC/NR1F3 Antibody (RORC/2941) [NBP3-08314] - IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using ROR gamma/RORC/NR1F3 antibody (RORC/2941) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 : HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/ROR-gamma-RORC-NR1F3-Antibody-RORC-2941-Immunohistochemistry-Paraffin-NBP3-08314-img0005.jpg "Immunohistochemistry-Paraffin: ROR gamma/RORC/NR1F3 Antibody (RORC/2941) [NBP3-08314] - IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using ROR gamma/RORC/NR1F3 antibody (RORC/2941) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 : HRP-polymer, 30min. DAB, 5min.")
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