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RhoA Antibody (1B3-4A10)

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Western Blot: RhoA Antibody (1B3-4A10) [NBP2-22529] - Analysis of RhoA/C was performed by loading 25 ug of various whole cell lysates or 0.5 ug of purified protein (RhoA, RhoB, and a GST-RhoC fusion) per well onto a ...read more
Immunocytochemistry/ Immunofluorescence: RhoA Antibody (1B3-4A10) [NBP2-22529] - Analysis of Rho A/C (green) showing staining in the in the cytoplasm of A431 cells (right) compared to a negative control without primary ...read more
Immunohistochemistry-Paraffin: RhoA Antibody (1B3-4A10) [NBP2-22529] - Analysis showing staining in the cytoplasm and membrane of human prostate carcinoma (right) compared with a negative control without primary ...read more
Immunocytochemistry/ Immunofluorescence: RhoA Antibody (1B3-4A10) [NBP2-22529] - Analysis of RhoA/C (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room ...read more
Immunocytochemistry/ Immunofluorescence: RhoA Antibody (1B3-4A10) [NBP2-22529] - Analysis of Rho A/C (green) showing staining in the in the cytoplasm of Hela cells (right) compared to a negative control without primary ...read more
Immunohistochemistry-Paraffin: RhoA Antibody (1B3-4A10) [NBP2-22529] - Analysis showing staining in the cytoplasm of human hepatocarcinoma (right) compared with a negative control without primary antibody (left).
Immunohistochemistry-Paraffin: RhoA Antibody (1B3-4A10) [NBP2-22529] - Analysis showing staining in the cytoplasm and membrane of mouse liver tissue (right) compared with a negative control without primary antibody ...read more
Immunoprecipitation: RhoA Antibody (1B3-4A10) [NBP2-22529] - Analysis of RhoA/C was performed on HeLa cells. Antigen-antibody complexes were formed by incubating 500ug of whole cell lysate with 2ug of a RhoA/C ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clone
1B3-4A10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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RhoA Antibody (1B3-4A10) Summary

Immunogen
Full-length human recombinant RhoA protein.
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
RHOA
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:20 - 1:200
  • Immunohistochemistry 1:50 - 1:500
  • Immunohistochemistry-Paraffin 1:50 - 1:500
  • Immunoprecipitation 2 ug
  • Western Blot 1:500 - 1:1500
Publications
Read Publication using NBP2-22529.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA and 30% glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for RhoA Antibody (1B3-4A10)

  • h12
  • oncogene RHO H12
  • ras homolog gene family, member A
  • Rho cDNA clone 12
  • RHO12
  • RhoA
  • RHOH12ARH12ARHAAplysia ras-related homolog 12
  • small GTP binding protein RhoA
  • transforming protein RhoA

Background

RhoA belongs to the small GTPase superfamily, Rho family. It regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesion and actin stress fibers. It serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. It binds PRKCL1, ROCK1 and ROCK2. Interacts with NET1 and ARHGEF3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RhoA Antibody (NBP2-22529)(1)

We have publications tested in 2 applications: B/N, WB.


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B/N
(1)
WB
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RhoA Antibody (NBP2-22529) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Research Areas for RhoA Antibody (NBP2-22529)

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Blogs on RhoA.

The role of Wnts in neuroinflammation
By Michalina Hanzel, PhDThe multifaceted roles of the Wnt family of glycoproteins have been extensively characterized throughout embryonic development and adult homeostasis. The highly conserved, cell- and tissue- s...  Read full blog post.

TGF-beta for treating degenerative intervertebral disc disease
By Jamshed Arslan Pharm.D. Our upright posture and balance depend on a jelly-like material, called nucleus pulposus (NP), in the middle of intervertebral discs. NP cells protect us from disc degeneration by maintain...  Read full blog post.

The use of actin as a loading control in research on fruiting-body development and vegetative growth in Sordaria macrospora research
Sordaria macrospora is a filamentous fungus that serves as very useful system for scientific research due to a short life cycle and easy manipulation.  Just like any other model organism, it is important to have an effective loading control to va...  Read full blog post.

HIF-1 beta - activating gene transcription in response to hypoxia
Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric transcription factor consisting of alpha and beta subunits. The levels of functional HIF-1 in the cell depends on the level of oxygen allowing cells to respond to hypoxic conditions. HIF-1a is a...  Read full blog post.

A good helper on validating your FLOW and IHC data - Rabbit IgG Isotype Control
Isotype controls are primarily used as negative controls in flow cytometry but they can also be used for immunohistochemistry. They are used to approximate the non-specific target primary antibody binding due to protein-protein interactions, binding t...  Read full blog post.

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Bioinformatics

Gene Symbol RHOA