![Western Blot: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of 25 ug of various whole cell lysates or 0.5 ug of purified protein (full-length His-RhoA, truncated GST-RhoB or full-length GST-RhoC fusion) per well onto a 4-20% Tris-HCl polyacrylamide gel.](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Western-Blot-NBP2-22528-img0001.jpg "Western Blot: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of 25 ug of various whole cell lysates or 0.5 ug of purified protein (full-length His-RhoA, truncated GST-RhoB or full-length GST-RhoC fusion) per well onto a 4-20% Tris-HCl polyacrylamide gel.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clone | 1A11-4G10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Full-length recombinant human Rho A |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RHOA |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS with 1 mg/ml BSA and 30% glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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![Immunocytochemistry/Immunofluorescence: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of Rho A/B/C (green) showing staining in the in the cytoplasm of A431 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Immunocytochemistry-Immunofluorescence-NBP2-22528-img0005.jpg "Immunocytochemistry/Immunofluorescence: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of Rho A/B/C (green) showing staining in the in the cytoplasm of A431 cells (right) compared to a negative control without primary antibody (left).")
![Immunohistochemistry-Paraffin: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis showing staining in the cytoplasm of human hepatocarcinoma (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Immunohistochemistry-Paraffin-NBP2-22528-img0008.jpg "Immunohistochemistry-Paraffin: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis showing staining in the cytoplasm of human hepatocarcinoma (right) compared with a negative control without primary antibody (left).")
![Immunocytochemistry/Immunofluorescence: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of RhoA/B/C (green) in NIH3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA/TBST for 15 minutes at room temperature. Cells were probed with a RhoA/B/C monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488-conjugated goat-anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Immunocytochemistry-Immunofluorescence-NBP2-22528-img0003.jpg "Immunocytochemistry/Immunofluorescence: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of RhoA/B/C (green) in NIH3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA/TBST for 15 minutes at room temperature. Cells were probed with a RhoA/B/C monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488-conjugated goat-anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.")
![Immunocytochemistry/Immunofluorescence: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of Rho A/B/C (green) showing staining in the in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Immunocytochemistry-Immunofluorescence-NBP2-22528-img0004.jpg "Immunocytochemistry/Immunofluorescence: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of Rho A/B/C (green) showing staining in the in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left).")
![Immunohistochemistry-Paraffin: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of showing staining in the cytoplasm of mouse breast tissue (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Immunohistochemistry-Paraffin-NBP2-22528-img0006.jpg "Immunohistochemistry-Paraffin: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of showing staining in the cytoplasm of mouse breast tissue (right) compared with a negative control without primary antibody (left).")
![Immunohistochemistry-Paraffin: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis showing staining in the cytoplasm of human prostate carcinoma (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Immunohistochemistry-Paraffin-NBP2-22528-img0007.jpg "Immunohistochemistry-Paraffin: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis showing staining in the cytoplasm of human prostate carcinoma (right) compared with a negative control without primary antibody (left).")
![Immunoprecipitation: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of RhoA/B/C was performed on HeLa cells. Antigen-antibody complexes were formed by incubating 750 ug of HeLa cell lysate with 2 ug of a RhoA/B/C monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50 ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Eluted sample (right lane) and 25 ug of HeLa cell lysate (left lane) were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a Rho A/B/C monoclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-blot IP Detection Reagent at a dilution of 1:2500 for at least 1 hour.](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Immunoprecipitation-NBP2-22528-img0002.jpg "Immunoprecipitation: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of RhoA/B/C was performed on HeLa cells. Antigen-antibody complexes were formed by incubating 750 ug of HeLa cell lysate with 2 ug of a RhoA/B/C monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50 ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Eluted sample (right lane) and 25 ug of HeLa cell lysate (left lane) were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a Rho A/B/C monoclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-blot IP Detection Reagent at a dilution of 1:2500 for at least 1 hour.")
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