Rheb Antibody (1E12)

Images

 
Western Blot: Rheb Antibody (1E12) [H00006009-M05] - Analysis of RHEB expression in transfected 293T cell line by RHEB monoclonal antibody (M05), clone 1E12. Lane 1: RHEB transfected lysatE (20.5 KDa). Lane 2: ...read more
ELISA: Rheb Antibody (1E12) [H00006009-M05] - Detection limit for recombinant GST tagged RHEB is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
1E12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Rheb Antibody (1E12) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
RHEB (AAH16155, 1 a.a. ~ 184 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPQSKSRKIAILGYRSVGKSSLTIQFVEGQFVDSYDPTIENTFTKLITVNGQEYHLQLVDTAGQDEYSIFPQTYSIDINGYILVYSVTSIKSFEVIKVIHGKLLDMVGKVQIPIMLVGNKKDLHMERVISYEEGKALAESWNAAFLESSAKENQTAVDVFRRIILEAEKMDGAASQGKSSCSVM
Specificity
Ras homolog enriched in brain
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
RHEB
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Western Blot 1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Rheb Antibody (1E12)

  • GTP-binding protein Rheb
  • MGC111559
  • Ras homolog enriched in brainRHEB2Ras homolog enriched in brain 2
  • Rheb
  • Rheb2

Background

This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RHEB
Entrez
OMIM
Uniprot