![Western Blot: RGS8 Antibody [NBP2-20153] - Decreased steady-state levels of Rgs8 message and protein in BAC-Q72 mice.Western blot analyses indicate reduction of Rgs8 steady-state levels in cerebella of BAC-Q72 mice, but no change in BAC-Q22 mice when compared with wild-type mice. The blot is a representative Western blot of 3 independently performed experiments with 2 animals each per BAC line. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1005182) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RGS8-Antibody-Western-Blot-NBP2-20153-img0002.jpg "Western Blot: RGS8 Antibody [NBP2-20153] - Decreased steady-state levels of Rgs8 message and protein in BAC-Q72 mice.Western blot analyses indicate reduction of Rgs8 steady-state levels in cerebella of BAC-Q72 mice, but no change in BAC-Q22 mice when compared with wild-type mice. The blot is a representative Western blot of 3 independently performed experiments with 2 animals each per BAC line. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1005182) licensed under a CC-BY license.")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human RGS8. The exact sequence is proprietary. |
| Predicted Species | Rat (98%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | RGS8 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 21 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for RGS8 Antibody (NBP2-20153)Find related products by research area.
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RNA-binding protein Staufen1 conspires with Atxn2 in stress granules to cause neurodegeneration by dysregulating RNA metabolism By Jamshed Arslan Pharm.D. Spinocerebellar ataxia type 2 (SCA2) is a movement disorder characterized by neurodegeneration. The cause of this autosomal dominant disease is a mutation in the RNA processing gene Atxn2,... Read full blog post. |
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![Western Blot: RGS8 Antibody [NBP2-20153] - Sample (30 ug of whole cell lysate) A: NT2D1 B: IMR32 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/RGS8-Antibody-Western-Blot-NBP2-20153-img0001.jpg "Western Blot: RGS8 Antibody [NBP2-20153] - Sample (30 ug of whole cell lysate) A: NT2D1 B: IMR32 12% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: RGS8 Antibody [NBP2-20153] - Overexpression of mutant ATXN2 in human SH-SY5Y cells recapitulates down-regulation of in vivo steady-state levels of Rgs8 in BAC-Q72 mice.Cells were transfected with plasmids encoding Flag-tagged cDNAs of human ATXN2 containing Q22 or Q58 or Q108 repeats. Forty-eight hrs post-transfection, cells were selected with hygromycin (40 µg/ml) for 5–7 days & hygromycin resistant cells were harvested as two aliquots. (A) Protein extracts were prepared from one aliquot & subjected to Western blot analyses to measure steady-state levels of RGS8. The blots were re-probed for beta -Actin as an internal loading control. (B) Quantitative RT-PCR analyses of synthesized cDNAs from the other aliquot demonstrate moderate reduction of RGS8 mRNA in cells expressing Flag-ATXN2-Q108. The data are means ± SD, *p<0.05. (C) Mutant ATXN2 specifically induces decrease of RGS8 expression. MYC-tagged RGS8 cDNA including 5’ & 3’ UTRs was cloned under the transcriptional control of the CMV promoter & transfected into short-term hygromycin selected SH-SY5Y cell lines expressing Flag-tagged ATXN2-Q22, -Q58 or -Q108. Forty-eight hrs post-transfection, levels of exogenous RGS8 are significantly decreased in cells expressing ATXN2-Q58 or -Q108 compared with cells expressing wild-type ATXN2-Q22. To control for equal transfection, we monitored levels of GFP, which was expressed as an independent cassette in the plasmid. Blots were re-probed for beta -actin as an internal loading control. The blot represents one of three independent experiments. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1005182), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-20153_rabbit-polyclonal-rgs8-antibody-310202416114723.jpg "Western Blot: RGS8 Antibody [NBP2-20153] - Overexpression of mutant ATXN2 in human SH-SY5Y cells recapitulates down-regulation of in vivo steady-state levels of Rgs8 in BAC-Q72 mice.Cells were transfected with plasmids encoding Flag-tagged cDNAs of human ATXN2 containing Q22 or Q58 or Q108 repeats. Forty-eight hrs post-transfection, cells were selected with hygromycin (40 µg/ml) for 5–7 days & hygromycin resistant cells were harvested as two aliquots. (A) Protein extracts were prepared from one aliquot & subjected to Western blot analyses to measure steady-state levels of RGS8. The blots were re-probed for beta -Actin as an internal loading control. (B) Quantitative RT-PCR analyses of synthesized cDNAs from the other aliquot demonstrate moderate reduction of RGS8 mRNA in cells expressing Flag-ATXN2-Q108. The data are means ± SD, *p<0.05. (C) Mutant ATXN2 specifically induces decrease of RGS8 expression. MYC-tagged RGS8 cDNA including 5’ & 3’ UTRs was cloned under the transcriptional control of the CMV promoter & transfected into short-term hygromycin selected SH-SY5Y cell lines expressing Flag-tagged ATXN2-Q22, -Q58 or -Q108. Forty-eight hrs post-transfection, levels of exogenous RGS8 are significantly decreased in cells expressing ATXN2-Q58 or -Q108 compared with cells expressing wild-type ATXN2-Q22. To control for equal transfection, we monitored levels of GFP, which was expressed as an independent cassette in the plasmid. Blots were re-probed for beta -actin as an internal loading control. The blot represents one of three independent experiments. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1005182), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")