![Immunohistochemistry: RGS5 Antibody (OTI1C1) [NBP2-00880] - Representative photomicrographs of hematoxylin and eosin and regulator of G-protein signaling (RGS) 5 in squamous cell carcinoma of the tongue in noninvasive and invasive portions. (a), (b), show H&E and RGS5 in noninvasive portions at 10x magnification. (f), (g), show H&E and RGS5 in noninvasive portions at 40x magnification. (k), (l) show H&E and RGS5 in invasive portions at 10x magnification. (p), (q), show H&E and RGS5 in invasive portions at 40x magnification. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/abs/10.1002/cre2.166) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RGS5-Antibody-OTI1C1-Immunohistochemistry-NBP2-00880-img0005.jpg "Immunohistochemistry: RGS5 Antibody (OTI1C1) [NBP2-00880] - Representative photomicrographs of hematoxylin and eosin and regulator of G-protein signaling (RGS) 5 in squamous cell carcinoma of the tongue in noninvasive and invasive portions. (a), (b), show H&E and RGS5 in noninvasive portions at 10x magnification. (f), (g), show H&E and RGS5 in noninvasive portions at 40x magnification. (k), (l) show H&E and RGS5 in invasive portions at 10x magnification. (p), (q), show H&E and RGS5 in invasive portions at 40x magnification. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/abs/10.1002/cre2.166) licensed under a CC-BY license.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | OTI1C1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Full length human recombinant protein of human RGS5 (NP_003608) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RGS5 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 20.8 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for RGS5 Antibody (NBP2-00880)Find related products by research area.
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![Western Blot: RGS5 Antibody (OTI1C1) [NBP2-00880] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RGS5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RGS5.](http://images.novusbio.com/fullsize/RGS5-Antibody-OTI1C1-Western-Blot-NBP2-00880-img0001.jpg "Western Blot: RGS5 Antibody (OTI1C1) [NBP2-00880] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RGS5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RGS5.")
![Flow Cytometry: RGS5 Antibody (OTI1C1) [NBP2-00880] - Flow cytometric Analysis of Jurkat cells, using anti-RGS5 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).](http://images.novusbio.com/fullsize/RGS5-Antibody-OTI1C1-Flow-Cytometry-NBP2-00880-img0004.jpg "Flow Cytometry: RGS5 Antibody (OTI1C1) [NBP2-00880] - Flow cytometric Analysis of Jurkat cells, using anti-RGS5 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).")
![Immunohistochemistry-Paraffin: RGS5 Antibody (OTI1C1) [NBP2-00880] - Staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RGS5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min)](http://images.novusbio.com/fullsize/RGS5-Antibody-OTI1C1-Immunohistochemistry-Paraffin-NBP2-00880-img0002.jpg "Immunohistochemistry-Paraffin: RGS5 Antibody (OTI1C1) [NBP2-00880] - Staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RGS5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min)")
![Flow Cytometry: RGS5 Antibody (OTI1C1) [NBP2-00880] - HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RGS5 antibody and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/RGS5-Antibody-OTI1C1-Flow-Cytometry-NBP2-00880-img0003.jpg "Flow Cytometry: RGS5 Antibody (OTI1C1) [NBP2-00880] - HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RGS5 antibody and then analyzed by flow cytometry.")
![Immunohistochemistry: RGS5 Antibody (OTI1C1) [NBP2-00880] - Representative photomicrographs of hematoxylin & eosin (H&E), CK5/6, regulator of G‐protein signaling (RGS) 5, N‐cadherin, vimentin, & E‐cadherin in squamous cell carcinoma of the tongue in noninvasive & invasive portions. (a), (b), (c), (d), & (e) show H&E, RGS5, N‐cadherin, vimentin, & E‐cadherin in noninvasive portions at 10× magnification. (f), (g), (h), (i), & (j) show H&E, RGS5, N‐cadherin, vimentin, & E‐cadherin in noninvasive portions at 40× magnification. (k), (l), (m), (n), & (o) show H&E, RGS5, N‐cadherin, vimentin, & E‐cadherin in invasive portions at 10× magnification. (p), (q), (r), (s), & (t) show H&E, RGS5, N‐cadherin, vimentin, & E‐cadherin in invasive portions at 40× magnification Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31049219), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-00880_mouse-rgs5-mab-oti1c1-310202415345344.jpg "Immunohistochemistry: RGS5 Antibody (OTI1C1) [NBP2-00880] - Representative photomicrographs of hematoxylin & eosin (H&E), CK5/6, regulator of G‐protein signaling (RGS) 5, N‐cadherin, vimentin, & E‐cadherin in squamous cell carcinoma of the tongue in noninvasive & invasive portions. (a), (b), (c), (d), & (e) show H&E, RGS5, N‐cadherin, vimentin, & E‐cadherin in noninvasive portions at 10× magnification. (f), (g), (h), (i), & (j) show H&E, RGS5, N‐cadherin, vimentin, & E‐cadherin in noninvasive portions at 40× magnification. (k), (l), (m), (n), & (o) show H&E, RGS5, N‐cadherin, vimentin, & E‐cadherin in invasive portions at 10× magnification. (p), (q), (r), (s), & (t) show H&E, RGS5, N‐cadherin, vimentin, & E‐cadherin in invasive portions at 40× magnification Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31049219), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")