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Relaxin-2 Antibody (349121) [Unconjugated]

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Relaxin‑2 was detected in immersion fixed LNCaP human prostate cancer cell line (positive staining) and HeLa human cervical epithelial carcinoma cell line (negative control) using Rat Anti-Human Relaxin‑2 Monoclonal Antibody (Catalog # MAB28041) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Relaxin‑2 was detected in immersion fixed LNCaP human prostate cancer cell line (positive staining) and HeLa human cervical epithelial carcinoma cell line (negative control) using Rat Anti-Human Relaxin‑2 ...read more
Relaxin‑2 was detected in immersion fixed paraffin-embedded sections of human prostate using Rat Anti-Human Relaxin‑2 Monoclonal Antibody (Catalog # MAB28041) at 15 µg/mL for 1 hour at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, ICC/IF
Clone
349121
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB28041-SP
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Relaxin-2 Antibody (349121) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Relaxin-2
Val23-Cys185
Accession # P04090
Specificity
Detects human Relaxin-2 in direct ELISAs.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Relaxin-2 Antibody (349121) [Unconjugated]

  • bA12D24.1.1
  • bA12D24.1.2
  • H2
  • relaxin 2
  • Relaxin2
  • Relaxin-2
  • RLN2
  • RLXH2

Background

Human Relaxin-2/H2 is a 6 kDa member of the insulin/relaxin gene superfamily. Relaxin-2 is synthesized as a 185 aa preprohormone that is proteolytically processed into a nonglycosylated, disulfide-linked, 53 aa mature heterodimer that contains a 29 aa B chain (aa 25‑53) and a 24 aa A chain (aa 161‑185). An alternate splice form of human Relaxin-2 is known that contains the B chain and an uncleaved 47 aa extension. Mature human Relaxin-2 heterodimer is 48%, 44% and 43% aa identical to rat, canine and porcine Relaxin-2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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